|1766-1||EVALUATION in vitro OF CELLULAR ACTIVATION INDUCED BY Ureaplasma diversum|
|Autores:||Lício Fábio Almeida Andrade Ferreira (UFBA - Universidade Federal da Bahia) ; Denisar Palmito (UFBA - Universidade Federal da Bahia) ; Jamile Siva Ramos (UFBA - Universidade Federal da Bahia) ; Percília Victória Oliveira (UFBA - Universidade Federal da Bahia) ; Tiana Baqueiro (UFBA - Universidade Federal da Bahia) ; Lucas Miranda Marques (UFBA - Universidade Federal da Bahia) ; Robson Amaro Augusto da Silva (UFBA - Universidade Federal da Bahia) |
Introduction: Ureaplasma diversum is an opportunistic pathogen that inhabits mucous membranes, whether respiratory or genitourinary tract. It has contributions in infections affecting cattle and goats. However, there are no reports of its presence in human disease. Among the diseases that can affect a flock, are the main granular vulvitis, abortion and infertility. The loss of animals by abortion is fact. The reduction in reproductive performance of cattle is related to reduced production, but even so, the mechanisms with which Ureaplasma diversum cause disease remain controversial. Few studies to understand the protective mechanisms of the host against infection by Ureaplasma diversum thus understanding the mechanisms that underlie the protection may facilitate the development of measures Immunoprophylactic. This study aims to evaluate the mechanisms of activating cell responses when in contact with Ureaplasma diversum. Materials and Methods: On that basis, we obtained cells from different body sites of C57BL/6 mice. Peritoneal, neutrophils and spleen macrophages were cultured over coverslips in RPMI medium. The different cells were infected with 104 CCU of Ureaplasma diversum and 24, 48 and 72 hours after infection supernatants were collected and stored at -20ºC. The cytokine production was evaluated by ELISA. Results: The interaction of different host cells with Ureaplasma diversum induces the release of proinflammatory mediators that may be related with cell activation and control of this pathogen. Conclusion: Our study has important data related to cellular responses to infection with Ureaplasma diversum. They may shed light on the understanding the innate immune response against Ureaplasma diversum infection and the proinflammatory molecules released in the interaction between pathogen and host cells. This new knowledge may contribute to develop new strategies of control of this pathogen and diminish farmer owners losses.
Keywords: Ureaplasma diversum; cell responses; cell culture; C57BL/6 mice; proinflammatory;ELISA.
Financial support: FAPESB, UFBA
Palavras-chave: Bactéria, C57BL/6 mice, Cultura de Células, Neutrófilo, Ureaplasma