Poster (Painel)
1621-1Characterization of endophytic bacteria for the potential use in agriculture
Autores:Vivian Jaskiw Szilagyi Zecchin (UFPR - Universidade Federal do Paraná) ; Angela Cristina Ikeda (UFPR - Universidade Federal do Paraná) ; Mariangela Hungria (EMBRAPA SOJA - Embrapa Soja) ; Douglas Adamoski (UFPR - Universidade Federal do Paraná) ; Vanessa Kava-cordeiro (UFPR - Universidade Federal do Paraná) ; Chirley Glienke (UFPR - Universidade Federal do Paraná) ; Lygia Vitória Galli-terasawa (UFPR - Universidade Federal do Paraná)


The literature shows that some bacteria, which live attached to plants, have the ability to promote plant growth. These microorganisms have attracted attention because of the need to reduce the use of chemicals, especially when looking at it in the context of sustainable agriculture and environmental. One of the strategies is to exploit the benefits microorganism action on the cultivation of plants, in the form of inoculants. The aim of this study was to characterize, by enzymatic analyzes and the physiological bacteria endophytic to the roots of corn (Zea mays L.) from LabGeM-UFPR (Universidade Federal do Paraná), that may have potential use in agriculture. Six endophytic bacteria of corn roots, five were Bacillus sp. and one Enterobacter sp. All of them were positive for the indication of nitrogen fixation by film formation, however, only LGMB128, LGMB163 and LGMB184 was the nifH gene amplified. Two strains of the genus Bacillus (LGMB128 and LGMB135), even with good responses to the production of IAA, siderophores and lytic enzymes, are not good candidates to act as growth promoters in a straightforward manner because seed germination is reduced. As an indication of favorable bacterial action, Enterobacter sp. LGMB184 and Bacillus sp. LGMB186`s root volume increased to 43.9% and 39.02% respectively, and seed germination to 47.0% and 55.5% respectively, even though not having presented the best results in biochemical tests. It is a highly specific effect, inducing an increase in the root absorption area, makes them potential candidates for future testing as biological inoculants for corn. Future studies are needed to test the biotechnological potential of these isolates in the field, in the hope that it will contribute as a means to help alternative sources in the cultivation of corn.

Palavras-chave:  Bacillus, corn, nifH, promoting plant growth, seed germination