XXI ALAM
Resumo:1596-2


Poster (Painel)
1596-2Bioprospecting of natural substances with activity against Candida albicans isolated from diabetic patients with periodontitis
Autores:Janaina de Cássia Orlandi Sardi (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho) ; Fernanda Patrícia Gullo (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho) ; Nayla de Souza Pitangui (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho) ; Thaís Bernardi (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho) ; Fernanda Sangalli-leite (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho) ; Luciana Arantes Soares (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho) ; Suélen Andreia Rossi (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho) ; Kaila Petrolina Medina Arlacón (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho) ; Maicon Segalla Petrônio (NUBBE - UNESP - Núcleo de Bioensaios, Biossíntesee Ecofisiologia) ; Luis Octávio Regasini (NUBBE - UNESP - Núcleo de Bioensaios, Biossíntesee Ecofisiologia) ; Dulce Helena Siqueira Silva (NUBBE - UNESP - Núcleo de Bioensaios, Biossíntesee Ecofisiologia) ; Vanderlan Bolzani (NUBBE - UNESP - Núcleo de Bioensaios, Biossíntesee Ecofisiologia) ; Ana Marisa Fusco-almeida (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho) ; Maria José Soares Mendes-giannini (UNESP - Universidade Estadual Paulista Júlio Mesquita Filho)

Resumo

Introduction: Candida albicans are commensal microorganisms that inhibit different sites of the oral cavity, including periodontal sites. In healthy subjects, these yeasts do not cause disease, however, in immunosuppressive conditions, such as diabetes mellitus, C. albicans can to become pathogenic. The study of plants as an alternative to the discovery of new drugs have great prominence because they are the best sources for obtaining a wide variety of drugs. Brazil has an enormous biodiversity, as well as a large territory with diversity of climate and soil condition that favors the development of rational and sustainable exploitation of new metabolites of therapeutic value. Objective: This study aimed to evaluate the antifungal activity of the natural compounds against isolates the Candida albicans from diabetic patients periodontitis. Methods: The antifungal activity of five derivate protocatechuic acids isolated Cupania oblongifolia, two fraction the Lippia sp and three derivate of caffeic acid was evaluated against ten clinical isolates of C. albicans. The determining the minimal inhibitory concentration (MIC) and minimal fungicide concentration (MFC) were evaluated according to EUCAST (European Committee on Antimicrobial Susceptibility Testing) document EDef7.2 (2008) with modifications. Results and Discussion: The results of bioprospecting antifungal showed that three of the five protocatechuic acid derivatives showed antifungal activity against isolates of C. albicans, and identified as octyl derivative which showed the best activity with an MIC ranging from 0.97 to 7.81 mg / L, but showed fungistatic activity with MFC value ranging from 15.62 to > 250 mg / L. The two fractions of Lippia spp did not show strong activity against isolates of C. albicans, with the exception of isolate 45, which showed a MIC and MFC of 15.62 mg / L. However to caffeic acid derivatives, all showed strong activity, the compound being identified as propyl cafeato presented the best activity with MIC ranging from 7.81 to 31.25 mg / L, considered fungistatic agent with MFC ranging from 62.25 to> 250 mg / L. Conclusion: Our results show that derivatives protocatechuic acid and caffeic acid, isolated from brazilian cerrado plants can be promising prototypes for the development of mouthwashes in patients with oral candidiasis caused by infection by C. albicans.


Palavras-chave:  Candida albicans, Antifungal activity, Bioprospecting