Resumo

Introduction: Cryptococcosis is an opportunistic mycosis and takes occupied the third place among the systemic fungal infections and also it is directly related to AIDS. Two pathogenic species, Cryptococcus neoformans and C. gattii affect immunocompromised and immunocompetent, respectively, developing clinical cases of meningitis, meningoencephalitis and pulmonary infections, whose treatment is performed with long-term amphotericin B and fluconazole. The infection begins with the process of adhesion the cells yeast in host cells. Thus, many virulence factors are studied from the adhesion process as well as many mechanisms of action of antifungal drugs are related to inhibition of adhesion. Objective: The main objective of this study was to evaluate the rate of adhesion in vitro of Cryptococcus neoformans in cell lines representing sites of infection and evaluate the anti-adhesion activity of the chalcone derivative. Methods: The evaluation of rate of compliance was achieved by plating for the recovery of infection colony members before and after contact with the substance chalcone derivative. The C. neoformans were maintained in contact with human cells for time interval between 0-48 h and after this, the cells were washed and detached using 1 mL trypsin. An aliquot was removed and plated on Sabouraud agar and incubated for 48 h. The number of adhered yeast in human cells was assessed by counting the CFU/mL. Results and Discussion: At the time of 1 h the rate of adhesion of C. neoformans showed greater adherence in most human cells tested. The cells of the central nervous system (U87) had a rate of 35% adhesion, showing the cell line with the highest preference, followed by metabolizing cells (HepG2) with 22% adhesion, keratinocytes (NOK) at 17%. At the time of 4 h was where higher membership in lung fibroblasts (MRC-5) with 5%, this being the cell line with lower adhesion capacity and a longer time to onset of infection. Conclusion: Our results have shown a predilection of C. neoformans cells to human glioma (U87), which agree with the data of onset of disease, since C. neoformans displays tropism for the central nervous system and chalcone can inhibit the uptake of the studied strains initial step for the establishment of infection. Regarding the chalcone can be considered an important prototype antifungal promising.