|1362-2||Composition and Diversity of Microbial Communities in Bulk Soil and Rhizosphere of sugarcane Grown in the State of São Paulo|
|Autores:||Diogo Paes da Costa (ESALQ-USP - Escola Superior de Acricultura "Luiz de Queiroz") ; Ademir Durrer Bigaton (ESALQ-USP - Escola Superior de Acricultura "Luiz de Queiroz") ; Thiago Gumieri (ESALQ-USP - Escola Superior de Acricultura "Luiz de Queiroz") ; Fernando Dini Andreote (ESALQ-USP - Escola Superior de Acricultura "Luiz de Queiroz") |
The metabolic peculiarity among rhizospherics environments is correlated with the differentiation of structure and composition of several microbiomes, including the bulk soil and native communities. The carbon balance in the rhizosphere can receive significant contribution of roots through the oozing, in addition to the organic compounds available via incorporation of MO in the fractions of the soil. Many studies of the composition and diversity of microbial communities associated with sugarcane that have observed the root exudation, the plant metabolism and the soil and climate characteristics have provided precious information through the metagenomics approaches. The aim of this study was to determine the composition and diversity of microbial communities present in the rhizosphere and bulk soil of plants of sugarcane grown in different areas of the State of São Paulo in Brazil. The plant samples were collected from seven regions and the rhizospheric and bulk soil were separated in the lab and submitted to DNA extraction procedure with commercial kit MoBio PowersoilTM. The gene sequences 16S and groups of Alfa and Betaproteobacteria were amplified with primers specific for subsequent application of the technique of denaturant gradient gel electrophoresis (DGGE). The DNA samples of the different groups were selected and subjected to large-scale sequencing through the platform of new generation Life Technologies Ion TorrentTM. The sequences obtained were analyzed through the tools of the Ribosomal Database Project (RDP) platform, making reference to the database available. The results showed a significant difference between the composition of microorganisms in the rhizosphere and bulk soil, while there was great similarity between groups of different areas sampled. The most representative classes were, respectively: Actinobacteria, Gamaproteobacteria, Bacilli, Alfaproteobacteria, Sphingobacteria and Betaproteobacteria (rhizosphere); and Acidobacteria, Actinobacteria, Alfaproteobacteria, Armatimonadia, Bacilli and Betaproteobacteria (bulk soil). The predominance of Proteobacteria in the rhizosphere highlighted the importance of this region in metabolic processes involved with cycling of C and N, highlighting the effect of the cultivation of sugarcane on the structures of microbial communities of cultivable soils.
Palavras-chave: Sugarcane, Microbial Communities, Rhizospheric environment, Metagenomic approache, Large-Scale Sequencing