XXI ALAM
Resumo:1282-1


Poster (Painel)
1282-1Antimicrobial activity of Chlorella vulgaris microalgae extract against Staphylococcus spp. isolated from mastitis-infected goat milk using three different methods
Autores:Anna Luiza Santana Neves (UFRPE - Universidade Federal Rural de Pernambuco) ; Pablo Eugênio da Costa E Silva (UFPE - Universidade Federal de Pernambuco / UFRPE - Universidade Federal Rural de Pernambuco) ; Noé Severino de Oliveira Neto (UFRPE - Universidade Federal Rural de Pernambuco) ; Carlos Rogério Ludolf de Andrade Lima Neto (UFRPE - Universidade Federal Rural de Pernambuco / UFRPE - Universidade Federal Rural de Pernambuco) ; Rinaldo Aparecido Mota (UFRPE - Universidade Federal Rural de Pernambuco) ; João Carlos Monteiro de Carvalho (USP - Universidade de São Paulo) ; Ana Lúcia Figueiredo Porto (UFRPE - Universidade Federal Rural de Pernambuco) ; Daniela de Araújo Viana Marques (UFRPE - Universidade Federal Rural de Pernambuco) ; Raquel Pedrosa Bezerra (UFRPE - Universidade Federal Rural de Pernambuco)

Resumo

Antibiotics administration is one of the most commonly used therapies to reduce the intramammary infections caused by pathogens in herds, and the most common reason for treatment was mastitis therapy. However, the uncontrolled use of these agents has led to appearance of microbial strains more resistant to classic antimicrobials. To overcome these problems, alternative natural antimicrobial products are investigated. Among these, microalgae become an attractive source of innovative classes of pharmacologically active natural compounds showing antibacterial, antiviral, antifungal, anticancer, anti-inflammatory and antimalarial activity. The aim of this work was study the antimicrobial activity of microalgae extract against bacteria isolated from mastitis-infected goat milk of Pernambuco State, Brazil. Chlorella vulgaris microalgae extract (50 mg/mL) obtained through sonication using 1M sodium acetate buffer have been tested in vitro against five bacteria of Staphylococcus spp. genera (namely, 1, 2, 3, 4 e 11D) using disk and cup-well diffusion agar techniques according to the guidelines of the National Committee for Clinical Laboratory Standards. All the isolates were resistant to microalgae extract using sodium acetate buffer. From these results, one isolate (bacteria 11D) was selected to analyze the antimicrobial activity using other assay type: spot-on-the-lawn with modifications. This test consists in a spotted of a putative amount of the microalgae extract over the agar medium containing Staphylococcus spp. bacteria streaked. Inhibition was detected by a zone of clearing around the place where it was spotted the microalgae extract. Staphylococcus spp. bacteria selected, 11D, was susceptibly to Chlorella vulgaris extract conducted by the spot-on-the-lawn method. This methodology is now being used in our laboratory to study antimicrobial activity of microalgae extracts against others animal ruminant mastitis pathogens.


Palavras-chave:  Antimicrobial activity, Chlorella vulgaris, goat mastitis, Staphylococcus spp.