|1199-1||Evaluation of nisin stability and activity into Bacterial Cellulose Membranes|
|Autores:||Angela Faustino Jozala (FCF/FBT/USP - Faculdade de Ciências Farmacêuticas. USP) ; Marcelo Akutagawa (FCF/FBT/USP - Faculdade de Ciências Farmacêuticas. USP) ; Paulo Vitor dos Santos Souza (FCF/FBT/USP - Faculdade de Ciências Farmacêuticas. USP) ; André Moreni Lopes (FCF/FBT/USP - Faculdade de Ciências Farmacêuticas. USP) ; Adalberto Pessoa Jr. (FCF/FBT/USP - Faculdade de Ciências Farmacêuticas. USP) |
There are important studies to achieve many bacterial cellulose (BC) products, but the production of it, with waste as a culture media and antimicrobial peptide incorporation are a novelty that has being exploited by our research group. The antimicrobial incorporation such as nisin or other drugs in bacterial cellulose has a wide applicability in the pharmaceutical-medical, chemical, cosmetic, food and among other areas. Nisin is a natural antimicrobial peptide used as food preservative; being effective at controlling a wide range of gram-positive bacteria, including multidrug-resistant pathogen. Bacterial cellulose (BC) is an extracellular polysaccharide produced by strains of the Gluconacetobacter xylinus. BC membranes production using waste as culture media is a novelty, stimulating scale-up for industrial production and extended applications in medical devices. The main goal of this work was evaluated nisin activity after BC membranes absorption. The standard nisin solution, 10 mL with 250 μg nisin/mL (Sigma®, 1g contain 2.5% of nisin), was prepared in phosphate buffer saline (PBS) pH 4.5 and sterilized by filtration, 0.22 μm. BC membranes were submerse in 1mL of nisin solution and the 24 wells-plate were incubated at 30oC under 100rpm for 4 hours. After absorption BC membranes were washed with sterilized water (MilliQ) and dried under 70oC for 10 minutes. Nisin activity was determined by agar diffusion assay with L. sakei as bioindicator. BC membranes were kept under refrigeration at 4oC and nisin activity was evaluated in different periods, 0, 1, 7, 14, 30 until 45 days. Results indicated nisin absorption after 4 hours in all BC membranes and were observed nisin activity improve, although from the nisin initial solution only 43% of proteins were transferred into the BC membranes. Stability results showed that BC membranes kept nisin activity and results should be highlighted, because an effective antimicrobial system able to assure safety and stability of pharmaceutical and medical products is extremely important. On a near future, the BC membranes with nisin will be tested against gram-positive and gram-negative microorganisms and its possible cytotoxicity effects, aim to prove this blend will be able to apply in wound healing.
Palavras-chave: bacterial cellulose, waste, nisin, G. xylinus, antimicrobial