|1122-2||STUDY OF THE MINIMUM INHIBITORY CONCENTRATION OF NISIN IMPREGNATED IN PLGA SCAFFOLDS|
|Autores:||Rafaela Coelho Correia (FCF/USP - Department of Biochemical and Pharmaceutical Technology) ; Angela Faustino Jozala (FCF/USP - Department of Biochemical and Pharmaceutical Technology) ; Kelly Fernanda Martins (PUC/SOROCABA - The Pontifical Catholic University) ; Thereza Christina Vessoni Penna (FCF/USP - Department of Biochemical and Pharmaceutical Technology) ; Eliana Aparecida de Rezende Duek (PUC/SOROCABA - The Pontifical Catholic University) ; Adalberto Pessoa Júnior (FCF/USP - Department of Biochemical and Pharmaceutical Technology) ; André Moreni Lopes (FCF/USP - Department of Biochemical and Pharmaceutical Technology) |
Introduction and Objectives
Nisin, an antimicrobial peptide, has also potential biomedical applications. Important advances in this field include the use of nisin in the development of antimicrobial packaging and liposome encapsulation. Research has also revealed the potential of nisin as a therapeutic agent, such as in cattle mastitis, human ulcer and topical skin infections. This bacteriocin when impregnated into the membranes of poly(lactic-co-glycolic acid) or PLGA may has a great therapeutic potential for scaffolds applications in skin infections combining two components into a single bioproduct.
Materials and Methods
The assays were carried out with PLGA membranes immersed in solution of nisin (PBS buffer/pH 4.5) in different concentrations: 250, 125, 62.5, 31.25, 15.63 and 7.81 μ/mL, and combined with EDTA at concentrations of 10, 20 and 40 mM. Then, these solutions were impregnated in PLGA membranes in sterile microtubes were centrifuged at 1.900xg for 1 minute, stirred and centrifuged again, the process was repeated 5 times, and remained in contact at 30ºC and 150 rpm until completely dry. Subsequently, the minimum inhibitory concentration (MIC) test was carried out added: culture medium, suspension of the microorganism to 106 UFC/mL of S. aureus or L. sakei, and then incubated for 24 hours. After that, was evaluated presence or absence of growth of microorganisms.
Results and Conclusions
According to the results, the combination of nisin/EDTA increased the inhibitory effect against Gram-positive bacteria. When tested in only nisin impregnated PLGA membranes were observed growth of S. aureus, in contrast there was inhibition of L. sakei with the MIC of nisin, 7.81 μg/mL. However, when combined nisin with EDTA there was inhibition of S. aureus, MIC of nisin was 62.5 μg/mL, and MIC of EDTA was 10 mM. The results demonstrate that the solutions of nisin/EDTA when impregnated in PLGA scaffolds may present great potential for application in the field of medical microbiology, in addition, we highlight that the next steps with study of the biomaterial should be carried out against Gram-negative.
Palavras-chave: nisin, PLGA, scaffolds, EDTA, drug delivery systems