|1026-1||ENZYMATIC ACTIVITY OF ENDOPHYTES FUNGI OF THE SEED
MACAÚBA (ACROCOMIA ACULEATA)
|Autores:||Elaine Rafaelle de Araújo Silva (UFPE - Universidade Federal de Pernambuco) ; Roberto dos Santos Siqueira (UFPE - Universidade Federal de Pernambuco) ; Idjane Santana de Oliveira (UFPE - Universidade Federal de Pernambuco) ; Romero Marinho de Moura (UFPE - Universidade Federal de Pernambuco) ; Laureen Michelle Houllou (CETENE - Centro de Tecnologias Estratégicas do Nordeste) |
The Macaúba (Acrocomia aculeata) is a species of palm found throughout Brazil. Its seed has a potential energy in biofuel production, and possible biotechnological applications of microorganisms comprising the microbiota of this palm. The study aimed to isolate endophytic fungi of macaúba and analyze its production capacity of amylase and lypase. Macaúba seeds were collected in a forest reserve in Recife - PE, Brazil. It was isolated for endophytes from the intact seeds and fragmented for the introduction of in vitro production of seedlings and free of microorganisms. The seeds were washed in tap water and commercial detergent for 5 minutes. Then the seeds were disinfected with 70% ethanol for 5 minutes, 5% NaClO for 10 minutes, 100% alcohol and flamed. The intact and fragmented seeds were introduced in the culture medium for fungi (PDA and CYA) and means for plants - Murashigue & Skoog (MS) medium plus 30 gL-1 sucrose, 0.1 gL-1 of myo-inositol , 0.5 mg l-1 thiamine, 0.5 mg l-1 nicotinic acid, 1 mg L-1 pyridoxine and 6 gL-1 agar. The culture media for fungi were incubated at 25 ° C for 7 days and the germination medium was kept in a growth chamber with a photoperiod of 16 h and controlled temperature of 25 + / - 2 ° C for up to 90 days. The isolates were screened for amylase and lypase in solid culture media containing soluble starch and Tween 20, respectively, as substrates for enzymes. The enzyme activity was measured by the diameter of the halo around the colony after 5 days. They were used as positive controls enzyme activity, a strain of Penicillium sp. and one Aspergillus sp. It was observed that in the culture media for fungi (PDA and CYA) no growth in the seed samples fragmented only on intact. And there was only one isolated fungus was identified belonging to the genus Aspergillus sp. This isolate was sown in the middle AFPA and presented the orange on the reverse of the colony, indicating that produce aflatoxin. The samples of seeds cultivating on MS medium were isolated from five molds (Mac 1, 2, 11, 13 and 14) respectively belonging to the genera: Penicillium sp, Sclerotium sp, Aspergillus sp., and also second Mac yeast. They were positive in the production of lypase: Mac 1 (1.0 cm) and Mac 14 (0.1 cm), and amylase, Mac 13 (0.3 cm) and 14 (0.2 cm). In general, all endophytic fungi showed low enzyme activity Macaúba purchased with controls.
Palavras-chave: Acrocomia Aculeata, Amylase, Lypase, Endophytes