|1016-1||MOLECULAR ANALYSIS OF RESISTANT MYCOBACTERIUM TUBERCULOSIS IN CLINICAL ISOLATES FROM SANTA CATARINA STATE, SOUTHERN BRAZIL.|
|Autores:||Rodrigo Ivan Prim (UFSC - Universidade Federal de Santa Catarina) ; Simone Senna (UFSC - Universidade Federal de Santa Catarina) ; Christiane Nogueira (UFSC - Universidade Federal de Santa Catarina) ; Marcos Schorner (UFSC - Universidade Federal de Santa Catarina) ; Luciana de Souza Nunes (HCPA/UFRGS - Hospital das Clínicas de Porto Alegre/) ; Darcita Buerger Rovaris (LACEN/SC - Laboratório Central de Saúde Pública de Santa Catarina) ; Maria Luiza Bazzo (UFSC - Universidade Federal de Santa Catarina) |
Introduction: Tuberculosis (TB) is a public health worldwide problem and Santa Catarina (SC), southern state of Brazil has one of the lowest national incidence rates (27.6/100,000). The Mycobacterium tuberculosis multidrug-resistant (MDR) strains emergence and spread has become a global threat. Santa Catarina state doesn't have a robust data about drug-resistant circulating strains, so the profile analysis will be important to understanding and tuberculosis control in SC.
Objective: This study aims to investigate the multiple drug resistance of Mycobacterium tuberculosis profile isolated from clinical samples from Santa Catarina State.
Methods: Ninety three M. tuberculosis strains were used is this study, which include 92 resistant isolates from clinical specimens and one H37Rv as sensitive standard strain. The stains were analyzed by Spoligotyping and sequencing in the Laboratory of Molecular Biology and Mycobacteria (LBMM) between 2010 and 2011. We sequence partial regions of genes katG, inhA, ahpC, rpoB and embC at Megabase 1000 sequencer.
Results: Among 92 strains, 53 (58%) were multidrug resistant. Between the resistant strains we found single mutations in in katG gene (A317P) and rpoB gene (S531L; D516V and I517F). Spoligotyping registers in SpolDB4 program and the LAM9 (29.8%) lineage was most frequently, followed by T2-T3 (15.8%), H3 (12.3%), U (LAM3?) (10.5%), LAM5 (3.5%), T1 (3.5%), T3 (3.5%), LAM2 (1.8%), LAM6 (1.8%), T2 (1.8%) and U (1.8%) and 12,3% strains were unknown and 1 orphan.
Conclusion: This study provided the molecular drug resistance analysis of M. tuberculosis in Santa Catarina. The findings were important to understanding the resistance mechanisms of circulating strains in the State, as well as the development and improve the new methods to resistance diagnosis.
Palavras-chave: Drug resistance, Tuberculosis, Sequencing