XXI ALAM
Resumo:935-1


Poster (Painel)
935-1Total proteomic analysis of proteins expressed by biofilm from Histoplasma capsulatum
Autores:Nayla de Souza Pitangui (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Janaína de Cássia Orlandi Sardi (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Liliana Scorzoni (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Julhiany de Fátima da Silva (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Fernanda Sangalli Leite (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Fernanda Patrícia Gullo (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Gabriela Rodriguez-arellanes (UNAM - Universidade Nacional Autonoma do México) ; Maria Lúcia Taylor (UNAM - Universidade Nacional Autonoma do México) ; Maria José Soares Mendes-giannini (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Ana Marisa Fusco-almeida (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho")

Resumo

Introduction: The pathogenic fungus Histoplasma capsulatum causes the respiratory and systemic disease named histoplasmosis. It is primarily acquired via aerosol exposure with the inhalation of its microconidia or hypha fragments. Evolution of respiratory disease depends on the ability of H. capsulatum yeasts to survive and replicate within alveolar macrophages. Later the infection of the lung, yeasts can disseminate to other organs of the body, including the spleen and liver, causing the most lethal form of the disease. Some microorganisms attached to biological and nonbiological surfaces are able to form biofilms, which are dynamic communities enclosed in an exopolymeric matrix. Biofilm formation by H. capsulatum was recently described by our group as a new virulence factor. Based on the importance of biofilms and its persistence on host tissues and cell surfaces, the present study was designed to investigate the expressed proteins by H. capsulatum biofilms and planktonic cells, and correlate with binding characteristics protein (adhesins). Materials and methods: H. capsulatum biofilm assays were performed in vitro using EH-315 strain, highly virulent. The proteomic analysis was performed by two-dimensional polyacrylamide gel electrophoresis including sample preparation, isoelectric focusing, electrophoretic separation and image scanning and analysis. The gels were analysed by software ImageMaster 2D Plattinum, allowing estimating the isoelectric points and molecular weights of expressed proteins. Results/Discussion: The proteomic analysis revealed that the samples exhibited different protein profiles, with an increased protein expression of the strain in biofilm compared to planktonic fungal growth, being approximately 250 proteins expressed exclusively by the fungus in biofilms, that will be sequenced by mass spectrometry to identify proteins with features of ligands that can act in the fungi interaction to host cells. Conclusion: The differential proteomic analysis revealed 250 proteins exclusively expressed by the fungus in biofilms and others with different levels of expression.


Palavras-chave:  Histoplasma capsulatum, Biofilm, Proteomic analysis, Virulence factor