Resumo

Fibrinolytic proteases are enzymes that degrade fibrin, the main component of blood clots. The accumulation of this protein leads to thrombosis responsible for cardiovascular disease including myocardial infarction. A promising alternative to thrombolytic therapy has been the production of these enzymes by microorganisms which promotes low cost, high efficiency and capacity for large scale production. Among the microorganism, filamentous fungi has been gaining attention because they are capable of producing extracellular proteases and a source bit being exploited for the production of fibrinolytic enzymes. This study aimed to select species of filamentous fungi isolated from soil of Caatinga - Pernambuco - Brazil and assess their potential for production of proteases with fibrinolytic activity using the medium for the fermentation of soy because it is a medium rich in proteins and low cost. Was used the medium 2% soybean. The fibrinolytic activity was performed by the spectrophotometric method and by degradation of the blood clot. Among the 36 isolates studied, 58% showed fibrinolytic activity above 100 U/mL. The microorganism with the higher activity in terms of enzyme production was Mucor subtilissimus SIS 42 who presented with 96 hours of fermentation fibrinolytic activity of 415 U/mL. Initially, the amount of protein present in the fermentation broth was 0.498 mg/ml and after 72 hours of fermentation 0.026 mg/mL showing the degradation of the protein exists in the production of proteases by the microorganism. At that time crude extract had a capacity of enzymatic degradation of the equine blood clots in 16.7%. The results are very attractive and are in agreement with the literature, these fungi are also little studied in the production of fibrinolytic proteases and are promising potential in the treatment of cardiovascular diseases.