|464-1||FUNGI ISOLATED FROM NORTHEAST REGION OF BRAZIL PRODUCERS OF TANNASES ENZYMES|
|Autores:||Camilla Savicius de Lima (UESC - Universidade Estadual de Santa Cruz) ; Ana Paula Trovatti Uetanabaro (UESC - Universidade Estadual de Santa Cruz) ; Andréa Miura da Costa (UESC - Universidade Estadual de Santa Cruz) |
Tannin Acyl Hidrolase (E.C.220.127.116.11), or tannase, hydrolyzes esters bonds of tannic acid, releasing glucose and gallic acid, they are produced by various microorganisms, and the filamentous fungi are the best producers. The tannases are widely used in food industries, helping to reduce the adverse effects of tannins, removing insoluble precipitates in wines and beers, in addition it can be applied in bioremediation of tannic effluent. The gallic acid formed by the action of tannase is an antioxidant compound used in the food and also pharmaceutical industry. The objective of this study was select new fungi that are good producers of tannases, with potential to biotechnology and industrial application, for this reason, we used 45 filamentous fungi isolated from the Northeast region of Brazil of geners: Trichoderma, Fusarium, Curvularia, Absidia, Aspergillus, Phaeosphaeria and Mucor. They were incubated in a Petri dish containing malt extract (15g/L), tannic acid (5 g/L) and agar (20 g/L) at 28 °C for up to 96h, and the fungi considered positive for the production of phenol oxidases were those that formed halos of brown color, and they had their halos measured every 24 hours. 09 of the 45 tested fungi produced the halo in the first 24 hours, among these 04 fungi of the genus Trichoderma sp. (0.9 - 1.3 cm), 01 Curvularia sp. (1.0 cm) and 04 fungi have not yet been identified (1.0 - 3.43 cm), the other 36 did not produce the brown halo until 96 h of cultivation. Therefore, it is believed that the fungus that presented the halo dark brown and with larger diameter in environment supplemented with tannic acid (3.43 cm), has a higher potential for tannase enzyme production. This result does not rule out the other 08 fungi, because they produced halos and brown coloration very intense when compared to each other. Thus, it is necessary the identification of the fungi and the confirmation of the capacity of tannase production by each pre-selected fungus, through submerged cultures and enzymatic analyzes and mass spectrometry analysis, because the tannase is an important enzyme of biotechnological and industrial application, and the discovery of new microorganisms that are good producers of this enzyme is very important.
Palavras-chave: BIOTECNOLOGY, ENZYMES, FUNGI, TANNASES