Poster (Painel)
365-2A culture medium of biomass production of Streptomyces sp. with PGPR activities
Autores:María Alejandra Ferreira-torres (PUJ-BOGOTÁ - UNIDIA, Pontificia Universidad Javeriana) ; Iván Camilo Ávila-cortes (PUJ-BOGOTÁ - UNIDIA, Pontificia Universidad Javeriana) ; Marcela Franco Correa (PUJ-BOGOTÁ - UNIDIA, Pontificia Universidad Javeriana) ; Ivonne Gutiérrez Rojas (PUJ-BOGOTÁ - UNIDIA, Pontificia Universidad Javeriana)


Residues from agroindustry have been widely used for design and optimization of culture media because they reduce cost of production and help with recycling of organic matter. These residues have been exploited to optimize enzymes and metabolites production media, but they have not been widely used for biomass production of actinobacteria with plant growth promotion potential. Our group has been working on this approach with promising results, but the downstream process increases its costs due to biomass-substrate separation step. To avoid this constraint we optimized a culture media for Streptomyces sp. (MCR26), an actinobacteria soil isolate with PGPR activities, using chemically defined carbon and nitrogen sources. We performed Crystal GP biochemical test to determine the carbohydrate assimilation profile of MCR 26. A simple statistic design, testing 20 carbon substrates, was carried out using a basal medium with minimal micronutrients and ammonium sulfate as nitrogen source (carbon contribution was adjusted to all substrates). The biomass production was evaluated by dry weight and a productivity analysis was completed to select two carbon sources, which provide the higher yield, choosing glucose and lactose. To optimize carbon sources concentration we performed a full factorial design with two central points, including yeast extract and ammonium sulfate as nitrogen sources. The higher biomass production was 3,41 g/L with lactose (30g/L) and yeast extract (4g/L) and 2,56 g/L with glucose (10 g/L), yeast extract (4g/L) and ammonium sulfate (4g/L). These results are being experimentally validated. Streptomyces sp. MCR 26 uses yeast extract as primary nitrogen source as well as micronutrients and vitamins source. Our results confirmed that simple carbon sources are suitable for media optimization avoiding elevated separation costs during the downstream process, which has a positive impact on massive biofertilizers production

Palavras-chave:  Residues from agroindustry, Streptomyces, PGPR activities, biomass production, fermentation