Resumo

Probiotics are products containg live microorganisms and when frequently ingested in appropriate amount bring benefits to the host, mainly to keep the gastrintestinal microbiota balance. The aim of the work was to evaluate the immunonodulatory effect of four strains of Lactobacillus in level of inteleukin-2 (IL-2), interleucin-10, interferon-γ (INF-γ) and immunoglobin-A (IgA) in intestinal mucosa and in blood of animals submitted to different treatments. For this, 76 male Swiss mouse, with 3 weeks of age, were distributed in six groups: Group 1 (control): 0,1 ml of saline solution (0,85%); Group 2: 0,1 ml of probiotic preparation formed by L. acidophilus ATCC 4356; Group 3: 0,1 ml of probiotic preparation formed by L. fermentum ATCC 9338; Group 4: 0,1 ml of probiotic preparation formed by L. rhamnosus ATCC 7469; Group 5: 0,1 ml of probiotic preparation formed by L. plantarum ATCC 8014; Group 6: 0,1 ml of probiotic preparation formed by a pool of Lactobacillus’ strains. Two animals of each group were sacrificed in experiment days 0, 15, 30, 45 and 60 to collect blood and bowel in order to perform analysis of IL-2, IL-10, IFN-γ in serum blood and in intestine contents. The results to IgA anlysis showed that animals of groups 2, 3 and 6 presented significantly greater concentration (P<0,05) of antibody in intestinal mucosa, when compared to control group, while animals of group 4 presented greater significance (P<0,01) according to Bonferroni test. For the concentration of IL-10 in serum the results showed no significant (ANOVA, Tukey and Bonferroni – P>0,05), between animals groups that receive probiotic and the control group, while intestine contents analysis showed greater significance in groups 3 (P<0,01) and 4 (P<0,01) according to Bonferroni test. The differences observed of IL-2 and IFN-γ concentrations in serum and intestinal mucosa showed no significance in groups that received probiotic, when compared to control group. Therefore the Lactobacillus strains evaluated were able to modulate the immune response in animal models suggest, promoting increase in levels of IL-10 and IgA in intestinal mucosa of animals that received differents probiotics preparations, with distinction to L. rhamnosus ATCC 7469 strain.