|298-1||Octyl gallate as prototypes sanitizing against Cryptococcus neoformans|
|Autores:||Fernanda Patrícia Gullo Gullo (UNESP - Universidade Estadual Paulista) ; Edson Maria Torres Torres (UNESP - Universidade Estadual Paulista) ; Maria José Soares Mendes-giannini Mendes-giannini (UNESP - Universidade Estadual Paulista) ; Valter Luiz Iost Teodoro Teodoro (UNESP - Universidade Estadual Paulista) ; Ana Marisa Fusco-almeida Fusco-almeida (UNESP - Universidade Estadual Paulista) ; Suélen Andreia Rossi Rossi (UNESP - Universidade Estadual Paulista) ; Vanderlan da Silva Bolzani Bolzani (UNESP - Universidade Estadual Paulista) ; Maicon Segalla Petrônio Petrônio (UNESP - Universidade Estadual Paulista) ; Dulce Helena Siqueira Silva Silva (UNESP - Universidade Estadual Paulista) |
Introduction: Of the 38 species of yeasts Cryptococcus two stand out epidemiologically, C. gattii and C. neoformans that affect immunocompetent and immunocompromised patients, respectively, and the main clinical manifestation is cryptococcal meningitis or meningoencephalitis. The species C. neoformans is the most prevalent world, particularly in regions with temperate and cold in urban areas, and the essential ecological niche, dried excreta of pigeons (Columbia livia). By the fact that C. neoformans be related to the environment, the use of sanitizing reduces cases of infection would be the environmental disinfection with sanitizing, which has fungicidal capacity. Furthermore, the arsenal of antifungal drugs and high toxicity of these make it necessary to find new molecules with antifungal activity aiming at the production of drugs. Objetives: The aim of this study was to evaluate the antifungal activity and cytotoxicity of synthetic molecules the octyl gallate isolate from Brazilian plant against the environmental isolates of C. neoformans. Methods: The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of octyl gallete, was evaluated according to the EUCAST document EDef7.2 (2008) with modifications. As a control we used the drug fluconazole and amphotericin B. The cytotoxicity assessment was done according to the MTT method using the cell line NOK (keratinocytes oral mucosa) and HepG2 (human hepatome). The strains used in the tests were C. neoformans ATCC 90012 and ATCC 24065, and ten environmental isolates of C. neoformans (VNI var. grubii) previously identified from the municipality of Araraquara /SP, belong the mycology collection of the Faculty of Pharmaceutical Sciences of Araraquara, UNESP. Results and Discussion: The octyl gallate showed strong antifungal activity against the environmental isolates of C. neoformans with MIC and MFC range varying from 0.48 to 1.95 mg/L. The assay of cytotoxicity octyl gallate has shown that the highest MIC (1.95 mg/L) showed 87.7% of cell viability for NOK and 93.3% cell viability for HepG2, indicating low cytotoxicity of this molecule. Conclusion: The results of this study suggests that octyl gallate is a bioactive molecule to the development of antifungal agents and sanitizing against environmental isolates the C. neoformans, since it has strong antifungal activity and low cytotoxicity.
Palavras-chave: Cryptococcosis, antifungal activity, cytotoxicity, octyl gallate