Poster (Painel)
Autores:Caroline Barcelos Costa Orlandi (FCFAR - UNESP - Universidade Estadual Paulista) ; Fernanda Campos de Medeiros (FCFAR - UNESP - Universidade Estadual Paulista) ; Fernanda Patrícia Gullo (FCFAR - UNESP - Universidade Estadual Paulista) ; Fernanda Sangalli Leite (FCFAR - UNESP - Universidade Estadual Paulista) ; Janaína de Cássia Orlandi Sardi (FCFAR - UNESP - Universidade Estadual Paulista) ; Luciana Arantes Soares (FCFAR - UNESP - Universidade Estadual Paulista) ; Maicon Segalla Petrônio (FCFAR - UNESP - Universidade Estadual Paulista) ; Dulce Helena Siqueira Silva (IQ-UNESP - Universidade Estadual Paulista) ; Vanderlan da Silva Bolzani (IQ-UNESP - Universidade Estadual Paulista) ; Luis Octávio Regasini (IQ-UNESP - Universidade Estadual Paulista) ; Maria Aparecida de Resende Stoianoff (ICB-UFMG - Universidade Federal de Minas Gerais) ; Ana Marisa Fusco Almeida (FCFAR - UNESP - Universidade Estadual Paulista) ; Maria José Soares Mendes Giannini (FCFAR - UNESP - Universidade Estadual Paulista)


Dermatomycosis are fungal infections involving the horny layer of the skin and appendages of people and animals. The fungi that most commonly cause cutaneous mycoses are the dermatophytes, which are commonly represented by the anamorphs of the genera Trichophyton, Microsporum and Epidermophyton and by yeasts of the genera Candida and Malassezia. Many antifungal drugs have been developed during the last two decades, but they are confined to a relatively few chemical classes, some of them exhibiting problems such poor efficacy, side effects, toxicity, drug interactions and development of resistance. These limitations emphasize the need to develop new and more effective antifungal agents and natural products are attractive prototypes for this purpose due to their broad spectrum of biological activities. Protocatechuic acid (3,4 - dihydroxybenzoic) is a derivative of benzoic acid found in fruits, nuts and vegetables. The presence of phenolic compounds has been widely studied for having pharmacological activities and also to inhibit lipid oxidation and fungi’s growth. This study aimed to evaluate the antifungal activity of two semi-synthetic derivates of prothocatecuic acid (PT09 and PT10) against clinical species of dermatophytes (T. rubrum 72, T. mentagrophytes 66 and Microsporum gypseum 180). The antifungal activity of protocatechuic acid was evaluated by determining the minimum inhibitory concentration (MIC), according to Clinical and Laboratory Standards Institute (CLSI), document M38-A2, with modifications. The minimal fungicidal concentration (MFC) was determined too. We performed a cytotoxicity assay with AMJ2-C11 cells (alveolar macrophages), following the methodology of tetrazolium salt (MTT) reaction. Both substances showed good activity against the three species of dermatophytes. The MIC and MFC of PT09 were 4 µg/mL for T. rubrum and T. mentagrophytes and 8 µg/mL for M. gypseum. PT10 showed a MIC and MFC of 4 µg/mL for T. rubrum and T. mentagrophytes and 15,6 µg/mL for M. gypseum. The cytotoxic analysis of the substances showed a cell viability of more than 50%. These results suggest that these semi-synthetic derivatives of protocatechuic acid may be a potential source of new agents for the treatment of dermatophytosis.

Palavras-chave:  Dermatophytosis, Microsporum gypseum, Protocatechuic acid, Trichophyton rubrum, T. mentagrophytes