|93-1||Detection of Ehrlichia sp. and Anaplasma sp. in Arthropods Collected at Rio De Janeiro state and other regions of Brazil.|
|Autores:||Daniele Np de Almeida (FIOCRUZ - Fundação Oswaldo Cruz) ; Tatiana Rozental (FIOCRUZ - Fundação Oswaldo Cruz) ; Alexsandra Rm Favacho (FIOCRUZ - Fundação Oswaldo Cruz) ; Adonai Alvino Pessoa-junior (FIOCRUZ - Fundação Oswaldo Cruz) ; Adauto Jg Araújo (FIOCRUZ - Fundação Oswaldo Cruz) ; Rosangela Zacarias Machado (UNESP - Universidade Estadual Paulista Júlio de Mesquita Filho) ; Teresa Cristina Monte Gonçalves (FIOCRUZ - Fundação Oswaldo Cruz) ; Jairo Dias Barreira (UNIRIO - Universidade Federal do Estado do Rio de Janeiro) ; Elba Regina Sampaio de Lemos (FIOCRUZ - Fundação Oswaldo Cruz) |
Rickettsiosis, diseases caused by proteobacteria widely distributed throughout the World, are zoonosis transmitted to humans by fleas, lice, ticks and mites. Since the 1930s, Brazilian spotted fever (BSF), caused by Rickettsia rickettsii, has been reported in Brazil with high fatality rate. However only recently other rickettsiosis “lato sensu”, such as ehrlichiosis and anaplasmosis, have been included in the list of tick-borne diseases of potential occurring in Brazil. About 580 arthropods collected from animals, humans and environment from different areas in the states of Rio de Janeiro, São Paulo, Piauí and Tocantins were taken to the Laboratory of Hantavirosis and Rickettsiosis, where were kept at -20ºC for further processing, after taxonomic identification. Among all processed arthropods, 203 were identified as Amblyomma cajennense; 112, Amblyomma sp.; 110, Rhipicephalus sanguineus; 53, Ornithodorus sp.; 24, Boophilus microplus; 21, Anocentor nitens; 09, Amblyomma cooperi; 04, Amblyomma aureolatum; 03 Amblyomma dubitatum; 38, Ctenocephalides felis and 03, Pediculus humanus. Ectoparasites were washed out using solution of sodium hypochlorite and 70% alcohol, and subsequently, their DNA were extracted using QIAamp DNA Blood Mini Kit Qiagen, following the manufacturer’s instructions. The polymerase chain reaction (PCR) was performed in 479 arthropods samples from Rio de Janeiro using specific pairs of primers for Ehrlichia canis, E. chaffeensis and Anaplasma phagocitophilum. 154 samples (60%) amplified 16S rRNA gene fragment of Ehrlichia species and 03 samples (1%) Anaplasma phagocitophilum. Samples of other states of Brazil are being submitted to PCR and all the amplicons will be sequenced and analyzed to characterize the resulting species. These preliminary results confirm the circulation of Rickettsia “lato sensu” agents in several arthropods in Rio de Janeiro by PCR. This study also serves as a warning for veterinarians and clinicians due to the presence of infected arthropods in pets bringing the possibility of infection in humans.
Palavras-chave: Ehrlichia, Anaplasma, Ticks, Epidemiological Surveillance