XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:174-2

Poster (Painel)

174-2

Paracoccidioides brasiliensis-reactive antibodies in patients with schistosomiasis

Authors:

Maria Claudia Noronha Dutra Menezes (UEL - Universidade Estadual de Londrina) ; Simone Cristina Castanho Babaine de Melo (SMS BANDEIRANTES - Secretaria Municipal de Saúde de Bandeirantes) ; Mari Sumigawa Kaminami (UEL - Universidade Estadual de Londrina) ; Francisco José de Abreu (UEL - Universidade Estadual de Londrina) ; Nilson de Jesus Carlos (UEL - Universidade Estadual de Londrina) ; Paula César Leonello (UEL - Universidade Estadual de Londrina) ; Luciene Airy Nagashima (UEL - Universidade Estadual de Londrina) ; Eiko Nakagawa Itano (UEL - Universidade Estadual de Londrina)

Abstract

Paracoccidioidomycosis is a disease caused by the fungus Paracoccidioides brasiliensis (Pb) and the efficient immune response is associated with Th1 pattern of cytokines. The chronic schistosomiasis disease caused by Schistosoma mansoni is characterized by a Th2 response. Both diseases can present as chronic form and often associated with rural area. Objective: The purpose of this work was to analyze the presence of the IgG anti-Paracoccidioides brasiliensis (Pb) in patients with schistosomiasis. Methodology: Serum from patients with chronic schistosomiasis (n = 100) were analyzed by ELISA, using Pb exoantigens (exoAg) and purified specific Pb 43 kDa glycoprotein (gp43). Cytokines (IFN-γ and IL-4) were measured in serum samples from: patients with schistosomiasis presenting positive reaction to Pb antigens (n=18), patients with schistosomiasis presenting negative reaction to Pb antigens (n = 9) and normal human serum (n = 9) by ELISA. Results: The results of ELISA with exoAg were positive in 18 samples (18%) and 14 samples with gp43 (14%). Elevated INF-γ serum level was observed in patients with schistosomiasis presenting positive reaction to Pb antigens in relation to others groups (p<0.05). Conclusion: The present study report for the first time the schistosomiasis cases with antibodies against specific Pb antigens and if is due to concomitant infection requires further investigation. Higher INF-γ serum levels in this group of patients suggest a predominantly Th1 response. ਀ Financial support: SETI/PR, Fundação Araucária, CNPq, CAPES, PROPPG/PROEX/UEL਀

Keyword: Paracoccidioidomycosis, Schistosoma mansoni, IFN-gamma, Th1, Cytokines

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