XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:174-1

Poster (Painel)

174-1

IgE and IgG anti-gp70 serum levels in human paracoccidioidomycosis

Authors:

Eiko Nakagawa Itano (UEL - Universidade Estadual de Londrina) ; Fabiana Felipin Rigobello (UEL - Universidade Estadual de Londrina) ; Audrey de Souza Márquez (UNOPAR - Universidade Norte do Paraná) ; Berenice Tomoko Tatibana (UEL - Universidade Estadual de Londrina) ; José Daniel Lopes (UNIFESP - Universidade Federal de São Paulo) ; Ayako Sano (CU - Chiba University) ; Mario Augusto Ono (UEL - Universidade Estadual de Londrina)

Abstract

The dimorphic fungus, Paracoccidioides brasiliensis (Pb), is the causative agent of paracoccidioidomycosis (PMC). The resistance in PCM is associated with cellular immune response (Th1), while susceptibility is related to the intense humoral immune response (Th2) with increase IgE levels. Considering the possibility of participation of gp70 of P. brasiliensis in the Th2 response, the present study was designed to determine human serum levels of IgE antibodies anti-gp70. P. brasiliensis(Pb339) somatic antigen (SA) was used as the source of antigen, once it showed the highest concentration of gp70. SA Ag samples were subjected to gel filtration chromatography (Sephadex G-150/120) for this analysis. Fractions containing gp70 detected by Western blot were re-fractionated by gel electrophoresis polyacrylamide (SDS-PAGE). Serum IgG and IgE anti-gp70 was determined by enzyme-linked immunosorbent assay with sera from chronic PCM patients (n=20) and normal donors (n=20). Additionally, were determined the serum levels of IgE anti-Exoantigen and total IgG, in 12 serum samples from patients with PCM, by ELISA, and then performed statiscall analysis using Pearson correlation test with IgE anti-gp70 level. The results showed significantly higher levels of IgE and IgG antibodies to g70 in the group of patients with PCM in relation of the control group (p<0.05). And the analysis of Pearson correlation showed a strong correlation between the level of IgE anti-gp70 and the level of IgE anti-exoantigen (r=0.7032) and the level of IgG anti-gp70 with the level total IgG (r=0.7542) in serum samples from patients with PCM. We conclude by the results that the increase in IgE levels suggests that gp70 has the potential to induce Th2 response, that require further study. ਀䘀椀渀愀渀挀椀愀氀猀甀瀀瀀漀爀琀㨀匀䔀吀䤀⼀倀刀Ⰰ 䘀甀渀搀愀漀䄀爀愀甀挀爀椀愀Ⰰ 䌀一倀焀Ⰰ 䌀䄀倀䔀匀Ⰰ 倀刀伀倀倀䜀⼀倀刀伀䔀堀⼀唀䔀䰀 ਀ ਀

Keyword: Somatic antigen, Th2, Antibodies, Paracoccidioides brasiliensis, Virulence factor

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