XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:170-2

Poster (Painel)

170-2

INFLUENCE OF THE HOST GENETIC BACKGROUND ON CYTOKINES EXPRESSION IN GRANULOMATOUS LESIONS OF P. BRASILIENSIS-INOCULATED INBRED MICE

Authors:

Eva Burger (USP - Universidade de Sao Paulo) ; Angela Satie Nishikaku (USP - Universidade de Sao PauloUNIFESP - Universidade Federal de Sao Paulo) ; Jose Vicente Alves (USP - Universidade de Sao Paulo) ; Raphael Fagnani Sanches Molina (USP - Universidade de Sao Paulo) ; Jacy Gameiro (UNICAMP - Universidade Estadual de Campinas) ; Cf Andrade (UNICAMP - Universidade Estadual de Campinas) ; Dagmar Stach-machado (UNICAMP - Universidade Estadual de Campinas) ; Zoilo Pires de Camargo (UNIFESP - Universidade Federal de Sao Paulo) ; Liana Verinaud (UNICAMP - Universidade Estadual de Campinas)

Abstract

The severe clinical forms of paracoccidioidomycosis present numerous granulomatous lesions and anergy in protective cellular immunity; in contrast, mild forms show few localized granulomas and preserved cellular immune response. This pattern can be reproduced by infecting susceptible (S) and resistant (R) mice with a virulent P. brasiliensis (Pb) isolate so allowing the comparison of the granulomas architecture, areas of lesions comprising Pb with preserved or altered morphology, and the presence and distribution of different cell populations, extracellular matrix components cytokines. Regarding these later components we studied relevant cytokines to granuloma formation and host-fungal interactions by determining their concentration in organ supernatant by ELISA, the expression of their mRNAs by RT-PCR and their localization by immunohistochemistry and compared the lesions developed in the omentum of S and R mice to detect eventual differences due to the genetic background of the mice. ਀吀栀攀琀漀琀愀氀愀爀攀愀猀漀昀氀攀猀椀漀渀猀愀猀眀攀氀氀愀猀琀栀攀瀀攀爀挀攀渀琀愀最攀漀昀琀栀攀猀攀氀攀猀椀漀渀猀漀挀挀甀瀀椀攀搀戀礀倀戀眀攀爀攀猀椀最渀椀昀椀挀愀渀琀氀礀栀椀最栀攀爀椀渀匀琀栀愀渀椀渀刀洀椀挀攀Ⰰ 椀渀瀀愀爀愀氀氀攀氀眀椀琀栀琀栀攀栀椀最栀攀爀瘀椀愀戀氀攀昀甀渀最愀氀氀漀愀搀猀椀渀琀栀攀昀漀爀洀攀爀猀琀爀愀椀渀⸀ The local production of stimulatory cytokines TNF-a and g-IFN was always higher in the R strain than in the S one; in contrast, the inhibitory cytokine TGF-b was detected in lower concentrations in the omental tissue of R mice than in those of the S mice, as indicated by the areas of positive imunohistochemical reactions.਀刀吀ⴀ倀䌀刀爀攀瘀攀愀氀攀搀愀渀㠀堀栀椀最栀攀爀攀砀瀀爀攀猀猀椀漀渀漀昀䤀䘀一ⴀ☀⌀㤀㐀㜀㬀洀刀一䄀愀渀搀愀渀㄀㄀堀栀椀最栀攀爀攀砀瀀爀攀猀猀椀漀渀漀昀吀一䘀ⴀ☀⌀㤀㐀㔀㬀洀刀一䄀椀渀刀琀栀愀渀椀渀匀猀琀爀愀椀渀愀渀搀椀洀洀甀渀漀栀椀猀琀漀挀栀攀洀椀猀琀爀礀猀栀漀眀攀搀琀栀愀琀琀栀攀渀甀洀戀攀爀漀昀䤀䘀一ⴀ☀⌀㤀㐀㜀㬀挀攀氀氀猀眀愀猀㈀⸀㔀堀栀椀最栀攀爀椀渀刀琀栀愀渀椀渀匀洀椀挀攀⸀ 䠀漀眀攀瘀攀爀Ⰰ 吀一䘀ⴀ☀⌀㤀㐀㔀㬀瀀漀猀椀琀椀瘀椀琀礀眀愀猀猀椀洀椀氀愀爀椀渀琀栀攀最爀愀渀甀氀漀洀愀猀漀昀匀愀渀搀刀洀椀挀攀⸀ 吀䜀䘀ⴀ☀⌀㤀㐀㘀㬀愀渀搀䤀䰀ⴀ㄀　洀刀一䄀猀眀攀爀攀爀攀猀瀀攀挀琀椀瘀攀氀礀㄀⸀㈀愀渀搀㄀⸀　洀漀爀攀攀砀瀀爀攀猀猀攀搀椀渀匀琀栀愀渀椀渀刀洀椀挀攀愀渀搀琀栀攀爀攀眀攀爀攀㄀⸀㈀堀洀漀爀攀吀䜀䘀ⴀ☀⌀㤀㐀㘀㬀挀攀氀氀猀椀渀琀栀攀昀漀爀洀攀爀猀琀爀愀椀渀⸀ 吀栀攀琀漀琀愀氀愀爀攀愀漀昀最爀愀渀甀氀漀洀愀琀漀甀猀氀攀猀椀漀渀猀愀渀搀琀栀攀爀攀氀愀琀椀瘀攀愀爀攀愀猀漀昀氀攀猀椀漀渀猀挀漀渀琀愀椀渀椀渀最倀戀眀攀爀攀爀攀猀瀀攀挀琀椀瘀攀氀礀㄀⸀㈀堀愀渀搀㄀⸀㤀堀洀漀爀攀攀砀琀攀渀猀椀瘀攀椀渀匀洀椀挀攀琀栀愀渀椀渀琀栀攀刀漀渀攀猀⸀ ELISA of omentum homogenates also revealed high production of regulatory IL-10 and TGF-β cytokines and low levels of stimulatory cytokines TNF-a and g-IFN.਀吀栀攀爀攀昀漀爀攀Ⰰ 椀渀昀攀挀琀椀漀渀漀昀刀洀椀挀攀戀礀愀瘀椀爀甀氀攀渀琀倀戀猀琀爀愀椀渀氀攀愀搀猀琀漀琀栀攀瀀爀攀昀攀爀攀渀琀椀愀氀猀礀渀琀栀攀猀椀猀漀昀洀刀一䄀猀愀渀搀漀昀瀀爀漀琀攀椀渀猀漀昀吀一䘀ⴀ☀⌀㤀㐀㔀㬀愀渀搀䤀䘀一ⴀ☀⌀㤀㐀㜀㬀琀栀愀琀瀀爀漀洀漀琀攀洀愀挀爀漀瀀栀愀最攀愀挀琀椀瘀愀琀椀漀渀Ⰰ 瀀爀漀戀愀戀氀礀攀渀栀愀渀挀椀渀最倀戀欀椀氀氀椀渀最愀渀搀挀漀渀琀爀漀氀漀昀昀甀渀最愀氀搀椀猀猀攀洀椀渀愀琀椀漀渀椀渀瀀愀爀愀氀氀攀氀眀椀琀栀搀攀瘀攀氀漀瀀洀攀渀琀漀昀猀洀愀氀氀最爀愀渀甀氀漀洀愀琀漀甀猀氀攀猀椀漀渀猀挀漀渀琀愀椀渀椀渀最昀攀眀昀甀渀最椀㬀眀栀攀爀攀愀猀椀渀昀攀挀琀椀漀渀漀昀匀洀椀挀攀攀氀椀挀椀琀猀瀀爀攀昀攀爀攀渀琀椀愀氀猀礀渀琀栀攀猀椀猀漀昀洀刀一䄀猀愀渀搀瀀爀漀琀攀椀渀漀昀吀䜀䘀ⴀ☀⌀㤀㐀㘀㬀愀渀搀䤀䰀ⴀ㄀　眀栀椀挀栀搀攀愀挀琀椀瘀愀琀攀洀愀挀爀漀瀀栀愀最攀猀愀渀搀洀愀礀椀渀栀椀戀椀琀倀戀欀椀氀氀椀渀最戀礀洀愀挀爀漀瀀栀愀最攀猀Ⰰ 昀愀瘀漀爀椀渀最昀甀渀最愀氀搀椀猀猀攀洀椀渀愀琀椀漀渀愀渀搀昀漀爀洀愀琀椀漀渀漀昀戀椀最最攀爀最爀愀渀甀氀漀洀愀琀漀甀猀氀攀猀椀漀渀猀⸀ ਀匀唀倀倀伀刀吀㨀䘀䄀倀䔀匀倀 ⌀ 　㘀⼀㘀　　㤀㄀ⴀ㘀 ☀ ⌀ 　㠀⼀㔀㠀㘀　㐀ⴀ　㬀䌀一倀焀㌀　㐀㘀㌀　⼀㈀　　㤀ⴀ㠀

Keyword: susceptibility and resistance, granulomatous lesions, cytokines

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