XI International Meeting on Paracoccidioidomycosis
Resume:162-1


Investigação
162-1CONVENTIONAL AND MOLECULAR DIAGNOSIS OF ENDEMIC AND OPPORTUNISTIC MYCOSES
Authors:Beatriz L. Gomez (CIB - Corporacion para Investigaciones Biologicas)

Abstract

The definitive diagnosis of invasive fungal infections is usually based upon the isolation and identification of a specific etiologic agent in culture, and/or on the microscopic demonstration of the etiologic agent in histopathologic or other clinical specimens. Immunological tests (antibody and antigen detection) provide rapid results compared with the time required for culture and identification of fungal pathogens, and consequently they are often used as a basis for decisions on management of patients with invasive fungal infections. Tests for the detection of fungal antibodies have long had an important role in the diagnosis of endemic fungal infections, such as histoplasmosis, coccidioidomycosis, and paracoccidioidomycosis, as well as the different forms of aspergillosis that occur in nonimmunocompromised individuals. However, these tests still remain less useful for the opportunistic infections, such as aspergillosis and candidiasis, which occur in immunocompromised individuals. Antigen detection has long been an established method for the diagnosis of cryptococcosis, and the last few years have seen the advent of similar approaches for the detection of histoplasmosis, paracoccidioidomycosis, and penicilliosis. Although the use of monoclonal antibodies has enabled antigen detection tests to be marketed for the diagnosis of aspergillosis and candidiasis, finding circulating antigens that are not transient in nature remains an elusive goal. ਀一攀眀 洀漀氀攀挀甀氀愀爀 琀攀猀琀猀 漀昀昀攀爀 最爀攀愀琀 栀漀瀀攀 昀漀爀 琀栀攀 爀愀瀀椀搀 搀攀琀攀挀琀椀漀渀 愀渀搀 椀搀攀渀琀椀昀椀挀愀琀椀漀渀 漀昀 搀椀昀昀椀挀甀氀琀ⴀ琀漀ⴀ挀甀氀琀甀爀攀 漀爀最愀渀椀猀洀猀Ⰰ 愀渀搀 昀漀爀 爀愀瀀椀搀 搀椀愀最渀漀猀椀猀 搀椀爀攀挀琀氀礀 昀爀漀洀 挀氀椀渀椀挀愀氀 猀愀洀瀀氀攀猀⸀ 䠀漀眀攀瘀攀爀Ⰰ 昀攀眀 䐀一䄀ⴀ戀愀猀攀搀 琀攀猀琀猀 愀爀攀 挀甀爀爀攀渀琀氀礀 愀瘀愀椀氀愀戀氀攀 昀漀爀 爀漀甀琀椀渀攀 挀氀椀渀椀挀愀氀 甀猀攀⸀  一甀洀攀爀漀甀猀 ᰀ椠渀ⴀ栀漀甀猀攀ᴀ†瀀爀漀琀漀挀漀氀猀 栀愀瘀攀 戀攀攀渀 搀攀瘀攀氀漀瀀攀搀 昀漀爀 琀栀攀 搀攀琀攀挀琀椀漀渀 漀昀 昀甀渀最愀氀 䐀一䄀 椀渀 挀氀椀渀椀挀愀氀 猀瀀攀挀椀洀攀渀猀Ⰰ 戀甀琀 琀栀攀爀攀 栀愀猀 戀攀攀渀 渀漀 猀琀愀渀搀愀爀搀椀稀愀琀椀漀渀 漀昀 琀攀猀琀 瀀爀漀挀攀搀甀爀攀猀⸀ 䤀渀 洀漀爀攀 爀攀挀攀渀琀 猀琀甀搀椀攀猀Ⰰ 最攀渀攀猀 猀瀀攀挀椀昀椀挀 昀漀爀 琀栀攀 昀甀渀最甀猀 漀昀 椀渀琀攀爀攀猀琀 愀爀攀 渀漀眀 戀攀椀渀最 挀栀漀猀攀渀 愀猀 倀䌀刀 琀愀爀最攀琀猀Ⰰ 琀栀甀猀 攀氀椀洀椀渀愀琀椀渀最 琀栀椀猀 猀瀀攀挀椀昀椀挀椀琀礀 瀀爀漀戀氀攀洀⸀ 唀渀琀椀氀 琀栀攀猀攀 洀漀氀攀挀甀氀愀爀 瀀爀漀挀攀搀甀爀攀猀 愀爀攀 猀琀愀渀搀愀爀搀椀稀攀搀 愀渀搀 挀漀洀洀攀爀挀椀愀氀椀稀攀搀Ⰰ 椀洀洀甀渀漀氀漀最椀挀愀氀 琀攀猀琀猀 眀椀氀氀 爀攀洀愀椀渀 攀猀猀攀渀琀椀愀氀 昀漀爀 爀愀瀀椀搀 搀椀愀最渀漀猀椀猀 漀昀 椀渀瘀愀猀椀瘀攀 昀甀渀最愀氀 椀渀昀攀挀琀椀漀渀猀⸀ ਀吀栀椀猀 挀漀甀爀猀攀 眀椀氀氀 爀攀瘀椀攀眀 琀栀攀 眀椀搀攀 爀愀渀最攀 漀昀 洀攀琀栀漀搀猀 渀漀眀 愀瘀愀椀氀愀戀氀攀 昀漀爀 琀栀攀 搀椀愀最渀漀猀椀猀 漀昀 攀渀搀攀洀椀挀 愀渀搀 漀瀀瀀漀爀琀甀渀椀猀琀椀挀 椀渀瘀愀猀椀瘀攀 昀甀渀最愀氀 椀渀昀攀挀琀椀漀渀猀 愀渀搀 眀椀氀氀 攀砀愀洀椀渀攀 琀栀攀 瀀攀爀猀瀀攀挀琀椀瘀攀猀 愀渀搀 昀甀琀甀爀攀 漀昀 渀攀眀 洀攀琀栀漀搀漀氀漀最椀攀猀⸀  ਀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀䬀攀礀眀漀爀搀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀䐀䤀䄀䜀一伀匀䤀匀Ⰰ 䌀伀一嘀䔀一吀䤀伀一䄀䰀 䐀䤀䄀䜀一伀匀䤀匀Ⰰ 䴀伀䰀䔀䌀唀䰀䄀刀 䐀䤀䄀䜀一伀匀䤀匀Ⰰ 䔀一䐀䔀䴀䤀䌀 䴀夀䌀伀匀䔀匀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀