ÿþ<HTML><HEAD><TITLE>XI International Meeting on Paracoccidioidomycosis</TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>XI International Meeting on Paracoccidioidomycosis</font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>Resume:155-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td><b>Investigação</b><br><table width="100%"><tr><td width="60">155-1</td><td><b>Comparative genomic studies of Emmonsia spp., Paracoccidioides brasiliensis and other Onygenales</b></td></tr><tr><td valign=top>Authors:</td><td><u>Oliver Keatinge Clay </u> (CIB - Corporacion para Investigaciones BiologicasUROSARIO - Universidad del Rosario) ; Emily Ann Whiston (UC BERKELEY - University of California, Berkeley) ; Juan Esteban Gallo (CIB - Corporacion para Investigaciones Biologicas) ; Jose Fernando Munoz (CIB - Corporacion para Investigaciones BiologicasUDEA - Universidad de Antioquia) ; Elizabeth Misas (CIB - Corporacion para Investigaciones BiologicasUDEA - Universidad de Antioquia) ; John Waldo Taylor (UC BERKELEY - University of California, Berkeley) ; Juan Guillermo Mcewen (CIB - Corporacion para Investigaciones BiologicasUDEA - Universidad de Antioquia) </td></tr></table><p align=justify><b><font size=2>Abstract</font></b><p align=justify class=tres><font size=2>Several of the human-pathogenic dimorphic fungi belong to the order Onygenales. Nine genera from this order (<i>Paraccoccidioides</i>, <i>Histoplasma</i>, <i>Blastomyces</i>, <i>Coccidioides</i>, <i>Uncinocarpus</i>, <i>Arthroderma</i>, <i>Trichophyton</i>, <i>Microsporum</i> and <i>Ascosphaera</i>) are already represented by genomes of one or more isolates that have been sequenced, assembled and/or annotated. All known <i>Paracoccidioides</i> isolates belong to an inner clade that includes the pathogenic genera <i>Blastomyces</i> and <i>Histoplasma</i> as well as the typically non-pathogenic genus <i>Emmonsia</i>. As no <i>Emmonsia</i> genome sequences were available, we sequenced the genomes of <i>Emmonsia parva</i> UAMH 139 and <i>Emmonsia crescens</i> UAMH 3008 using Illumina paired-end, 101-bp read technology (one insert size) and are currently assembling, annotating, and analyzing them. Our initial de novo assembled scaffolds of the two <i>Emmonsia</i> species indicate that <i>E. crescens</i> UAMH 3008 and especially <i>E. parva</i> UAMH 139 are closer to <i>Blastomyces dermatitidis</i> than to other fully sequenced fungal species, in agreement with some earlier studies based on single genes. Thus, the coverage, syntenic regions and proximity should allow direct reference annotation of the <i>E. parva</i> protein-coding genes that are also present in <i>B. dermatitidis</i>. In the interest of identifying and understanding virulence factors and phase transitions in <i>Paracoccodioides brasiliensis</i>, our ultimate task is to compare the <i>E. parva</i> and <i>E. crescens</i> sequences to other Onygenales genomes within and beyond the inner clade, in a search for functionally relevant differences between the essentially non-pathogenic <i>Emmonsia</i> genomes and <i>P. brasiliensis</i> or other closely related pathogen genomes. In this presentation we discuss first results based on our initial working scaffolds, direct short read analyses, and ortholog alignments for selected genes.</font></p><br><b>Keyword: </b>&nbsp;comparative genomics, dimorphic fungi, Onygenales, bioinformatics, short read sequencing</td></tr></table></tr></td></table></body></html>