XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:144-1

Poster (Painel)

144-1

Comparison between ELISA and intradermoreaction tests in Paracoccidioidomycosis

Authors:

Tânia Regina Grão Velloso (UFES - Universidade Federal do Espirito Santo) ; Evandro Monteiro de Sá Magalhaes (UNIFAL-MG - Universidade Federal de Alfenas) ; Mayra Maciel Mendes Pereira (UNIFAL-MG - Universidade Federal de Alfenas) ; Marcelle Viçoso dos Santos (UNIFAL-MG - Universidade Federal de Alfenas) ; Rita de Cassia Elaine Pecci (UNIFAL-MG - Universidade Federal de Alfenas) ; Maria Rita Rodrigues (UNIFAL-MG - Universidade Federal de Alfenas) ; Sônia de Souza Lima Paes (S.M.S.A - Secretaria Municipal de Saúde de Alfenas) ; Zoilo Pires de Camargo (UNIFESP - Universidade Federal de São Paulo) ; Luiz Cosme Cotta Malaquias (UNIFAL-MG - Universidade Federal de Alfenas)

Abstract

Paracoccidioidomycosis (PMC) is a serious deep mycosis caused by the fungus dimorphic Paracoccidioides brasiliensis (Pb). Pb is a non-sexuated fungus, thermal dimorphic, which grows at 37°C in the form of yeast and in ambient temperature, shows itself in the form of thin filaments septats that originate the mycelia found in soil as permanent saprobe which is the infecting form. The disease can be classified into the chronicle or widespread form, also known as "adult", and in the acute or sub-acute form, "juvenile" type. The first is characterized by pulmonary commitment, ulceration of the skin, mucous membranes and lymphadenopathy, and could disseminate to all viscera's. The occurrence from the second is rare, compromising the fagocitical-mononuclear system and leads to bone marrow dysfunction. The disease is confined to the American continent, being Brazil the country with the largest number of cases and the Southern region of the State of Minas Gerais is considerated an endemic area. Thus, the goal of the work was essentially to verify the applicability of ELISA and double Immunodiffusion tests in early diagnosis of PCM. Furthermore, individuals of some rural communities of the municipality of Alfenas underwent intradermal test (IT) for PCM, interpreted by reading the diameter of induration. Also, it was made a complete blood count, clinical examination, thorax x rays, clinical exams and ELISA. The correlation between IT and ELISA positive shows moderate agreement, while the correlation between IT and ELISA negative IT positive and ELISA positive and IT negative and ELISA positive and IT positive e ELISA negative shows slight according. The agreement between ELISA and IT was 56% suggesting a slight to moderate agreement. From the data obtained, the correlation between sink positive test and positive ELISA testing has been evaluated so that they can eventually be used as a criterion for early diagnosis for PCM. This way, the results obtained according to the Kappa statistical method suggests a future alternative for this early diagnosis.

Keyword: ELISA, Intradermal test, Paracoccidioidomycosis, Diagnosis

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