XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:142-3

Poster (Painel)

142-3

Tracking Paracoccidioides lutzii among clinical isolates of Paracoccidioidomycosis in endemic areas of Brazil

Authors:

Fabiana Freire Mendes de Oliveira (UNB - Universidade de Brasilia) ; Rosane Cristine Hahn (UFMT - Universidade Federal de Mato Grosso) ; Marilene Chang (UFMS - Universidade Federal de Mato Grosso do Sul) ; Eduardo Honda (CEMETRON - Centro de Medicina Tropical de Rondônia) ; Mario Leon Silva-vergara (UFTM - Universidade Federal do Triangulo Mineiro) ; Maria Sueli Felipe (UNB - Universidade de Brasilia) ; Marcus Teixeira (UNB - Universidade de Brasilia)

Abstract

Paracoccidioidomycosis (PCM) is a chronic human disease caused by dimorphic fungal species from Paracoccidioides genus. Paracoccidioides brasiliensis was considered the unique species for many years however, multi locus sequencing typing, comparative genomics, evolutionary analysis and morphological characteristics revealed a highly degree of genetic divergence in this genus leading us to describe the new species Paracoccidioides lutzii. P. lutzii can be easily discriminated from P. brasiliensis by a large number of polymorphisms and by specific molecular markers, i.e., one INDEL of the hsp70, SNP's in gp43, arf and PRP8 genes. Comparative studies between the sibling species P. brasiliensis and P. lutzii may have significant clinical consequences on the diagnosis and therapeutics of PCM. This new species were already detected in Ecuador and Brazil in Goiás, Mato-Grosso states and single cases were detected in Roraima and Paraná states. We extended our analyses of clinical samples using molecular and morphological data in other geographical areas sampled and no sampled in Brazil such as Goiás, Mato-Grosso, Mato-Grosso do Sul, Rondônia and Minas Gerais states. Phylogenetic trees inferred by arf and gp43 loci revealed the presence of P. lutzii species in Rondônia and Minas Gerais where this species had never been described before. In addiction all isolates analysed from Goiás and Mato-Grosso states were also placed in the P. lutzii species reforcing the high frequency of P. lutzii in the Central-West part of Brazil. Conidia cells from these new strains also shares the elongated form of the standard strain Pb01 indicating that this morphological characteristic is shared in P. lutzii population. These studies are extremely important to epidemiology of PCM delineating the species boundaries in Paracoccidioides genus.

Keyword: Paracoccidioides lutzii, Epidemiology, Phylogeny

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