XI International Meeting on Paracoccidioidomycosis
Resume:126-2


Poster (Painel)
126-2Species distribution and antifungal susceptibility profile of oral Candida isolates from HIV-infected individuals.
Authors:Daniela Vanessa Moris (FMB - UNESP - Faculdade de Medicina deBotucatu - UNESP) ; Marcia Sc Melhem (IAL - SÃO PAULO - Instituto Adolfo Lutz) ; Marilena A Martins (IAL - SÃO PAULO - Instituto Adolfo Lutz) ; Maria Walderez Szeszs (IAL - SÃO PAULO - Instituto Adolfo Lutz) ; Lenice R Souza (FMB - UNESP - Faculdade de Medicina deBotucatu - UNESP) ; Lidia Raquel Carvalho (IBB - UNESP - Instituto de Biociências - UNESP) ; Rinaldo Poncio Mendes (FMB - UNESP - Faculdade de Medicina deBotucatu - UNESP)

Abstract

Oropharyngeal candidiasis continues to be a common opportunistic infection in the oropharynge of patients infected by the human immunodeficiency virus. Although Candida albicans remains the most common causative agent, other non-albicans species have been also identified. The aims of this study were to evaluate the prevalence of the different Candida spp in the oral cavity of HIV infected individuals and to determine the minimal inhibitory concentration to four antifungal compounds. A total of 300 samples were isolated and evaluated as to susceptibility to four antifungal compounds using the microdilution method as to AFST/EUCAST. C. albicans isolates were phenotypically and genotypically identified. methods; for Candida non-albicans species only phenotical methods were used. Comparison of frequencies was carried out by the qui-square test and significance was set at p<0.05. Prevalence of C. albicans was 89.0%, C. glabrata 6.0%, C. tropicalis 4.3% and C. krusei 0.7%. Prevalence of resistance and susceptibility dose dependent, taken together, to fluconazole was 0.75% in C. albicans, 50.0% in C. glabrata, 0.0% in C. tropicalis and 100.0% in C. krusei [C.glabrata>(C. albicans=C. tropicalis)]; as to ketoconazole, it was 0.75% in C. albicans, 0.0% in C. tropicalis, 0.0% in C. glabrata, and 50% in C. krusei [C. albicans=C. glabrata= C. tropicalis]; as to itraconazole, 1.9% in C. albicans, 72.2% in C. glabrata, 0.0% in C. tropicalis, and 50.0% in C. krusei [higher incidence of resistance in C. glabrata]; as to amphotericin B, all the isolates were susceptible. Cross-resistance to the three azole compounds was observed in two C. albicans and one C. krusei. Cross-resistance to two azole compounds was showed by one C. krusei and nine C. glabrata isolates. Our results show predominance of C. albicans in the oral cavity of HIV positive individuals, the presence of cross-resistance to azoles compounds, and a prevalence of resistance that suggests the evaluation of antifungal susceptibility testing whenever antifungal treatment with azoles is planned.਀ ਀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀䬀攀礀眀漀爀搀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀愀渀琀椀昀甀渀最愀氀 猀甀猀挀攀瀀琀椀戀椀氀椀琀礀Ⰰ 䠀䤀嘀ⴀ椀渀昀攀挀琀攀搀 椀渀搀椀瘀椀搀甀愀氀猀Ⰰ 䌀愀渀搀椀搀愀 猀瀀瀀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀