ÿþ<HTML><HEAD><TITLE>XI International Meeting on Paracoccidioidomycosis</TITLE><link rel=STYLESHEET type=text/css href=css.css></HEAD><BODY aLink=#ff0000 bgColor=#FFFFFF leftMargin=0 link=#000000 text=#000000 topMargin=0 vLink=#000000 marginheight=0 marginwidth=0><table align=center width=700 cellpadding=0 cellspacing=0><tr><td align=left bgcolor=#cccccc valign=top width=550><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=3><font size=1>XI International Meeting on Paracoccidioidomycosis</font></font></strong><font face=Verdana size=1><b><br></b></font><font face=Verdana, Arial,Helvetica, sans-serif size=1><strong> </strong></font></font></td><td align=right bgcolor=#cccccc valign=top width=150><font face=arial size=2><strong><font face=Verdana, Arial, Helvetica, sans-serif size=1><font size=1>Resume:112-1</font></em></font></strong></font></td></tr><tr><td colspan=2><br><br><table align=center width=700><tr><td><b>Poster (Painel)</b><br><table width="100%"><tr><td width="60">112-1</td><td><b>Sporotrichosis due to Sporothrix globosa in Brazil: Phenotypic, genotypic and serological characterization</b></td></tr><tr><td valign=top>Authors:</td><td><u>Anderson Messias Rodrigues </u> (UNIFESP - Federal University of São Paulo) ; Henri Donnarumma Levy Bentubo (UNIFESP - Federal University of São Paulo) ; Priscila Oliveira dos Santos (UNIFESP - Federal University of São Paulo) ; Cristiane Candida do Amaral (UNIFESP - Federal University of São Paulo) ; Alexandre Augusto Sasaki (UNIFESP - Federal University of São Paulo) ; Geisa Ferreira Fernandes (UNIFESP - Federal University of São Paulo) ; Olga Fischman Gompertz (UNIFESP - Federal University of São Paulo) ; Zoilo Pires de Camargo (UNIFESP - Federal University of São Paulo) </td></tr></table><p align=justify><b><font size=2>Abstract</font></b><p align=justify class=tres><font size=2>Sporotrichosis is a chronic mycotic infection caused by the traumatic implantation of pathogenic fungus involving the skin and subcutaneous tissue. This disease has been attributed to a single pathogen, <i>Sporothrix schenckii</i>. However, isolates identified as <i>S. schenckii</i> showed a great deal of phenotypic and genetic variability, suggesting that this taxon is a species complex. <i>Sporothrix globosa</i> is a widespread fungus. In this work we characterized a <i>S. globosa</i> isolate provided from a 66 years old male patient, from Minas Gerais state, Brazil. The patient presented a verrucous lesion on board, erythematous scaly-looking, with irregular borders and no sign of ulceration or suppuration were observed. For phenotypic characterization we evaluated the vegetative growth on PDA at different temperatures (30, 35 e 37 ºC), the colony colors on CMA, the assimilation pattern of carbon source (rafinose, ribitol and sucrose) and microscopic features of in vitro cultivation. At 25ºC colonies were moist, glabrous surface, cream to dark brown. At 37ºC, after 21 days, the isolate showed restricted growth. Microscopically the sessile conidia were brown, usually globose to subglobose. The isolate did not assimilate raffinose and ribitol as the only source of carbon. For molecular characterization, an 800 bp calmodulin gene fragment was amplified from genomic DNA using the primers CL1 and CL2A and then sequenced. GenBank search revealed that our isolate showed a 100% match with the CAL sequence of a Spanish isolate of <i>S. globosa</i> (FMR 8598), corroborating our phenotypic data. For serological characterization we employed the exoantigen from mycelial phase and whole cellular proteins from yeast phase in a Western blot assay to identify antigens recognized by IgG antibodies present in the patient serum. Western blot analyses showed a high reaction with the bands of 38 e 20 kDa in the exoantigen and 60, 50, 45, 38 e 30 kDa in whole cellular antigens. This case demonstrates the presence of S. globosa in Minas Gerais state in Brazil, and that the ability to differentiate among species leads to a more accurate estimation of fungal diversity. Moreover, surveillance of subcutaneous mycoses directly depends upon the accuracy of identification of the suspected infectious agent. This study was supported by FAPESP, CNPq and CAPES</font></p><br><b>Keyword: </b>&nbsp;Sporothrix, Sporothrix globosa, Characterization, Taxonomy, Serology</td></tr></table></tr></td></table></body></html>