XI International Meeting on Paracoccidioidomycosis
Resume:103-1


Investigação
103-1Paracoccidioides brasiliensis-induced migration of respiratory dendritic cells and subsequent antigen presentation in the lung draining lymph node
Authors:Sandro Almeida (USP - Universidade de Sao Paulo)

Abstract

Paracoccidioidomycosis is a mycotic disease caused by a terminally dimorphic fungus, Paracoccidioides brasiliensis (Pb), that starts with inhalation of the fungus; thus, lung cells such as DC are part of the first line of defense against this microorganism. Dendritic cells (DC) are antigen-presenting cells that act as sentinels in peripheral tissues, constantly sampling the antigens in their environment. Lung DC play a pivotal role in infections caused by airborne pathogens such as Mycobacterium tuberculosis, Aspergillus fumigatus and Cryptococcus neoformans. The DC population lining the lungs is key to the initiation of T cell responses after pulmonary challenge. DC remain quiescent until activated, at which point they migrate to the draining lymph nodes (DLN), present antigen, and initiate T cell activation.਀䈀攀挀愀甀猀攀 䐀䌀 愀爀攀 琀栀攀 洀漀猀琀 攀昀昀攀挀琀椀瘀攀 愀渀琀椀最攀渀 瀀爀攀猀攀渀琀椀渀最 挀攀氀氀猀 ⠀䄀倀䌀⤀ 昀漀爀 椀渀搀甀挀椀渀最 挀攀氀氀ⴀ洀攀搀椀愀琀攀搀 椀洀洀甀渀攀 爀攀猀瀀漀渀猀攀猀Ⰰ 椀琀 椀猀 椀洀瀀漀爀琀愀渀琀 琀漀 椀渀瘀攀猀琀椀最愀琀攀 氀甀渀最 䐀䌀 愀渀搀 琀栀攀椀爀 瀀漀琀攀渀琀椀愀氀 昀漀爀 氀礀洀瀀栀 渀漀搀攀 洀椀最爀愀琀椀漀渀 愀渀搀 椀洀洀甀渀攀 爀攀猀瀀漀渀猀攀 椀渀椀琀椀愀琀椀漀渀⸀ 吀栀攀 洀攀挀栀愀渀椀猀洀猀 椀渀瘀漀氀瘀攀搀 椀渀 爀攀猀椀猀琀愀渀挀攀 琀漀 倀戀 椀渀昀攀挀琀椀漀渀 愀爀攀 瀀漀漀爀氀礀 甀渀搀攀爀猀琀漀漀搀Ⰰ 戀甀琀 椀琀 椀猀 氀椀欀攀氀礀 琀栀愀琀 䐀䌀 瀀氀愀礀 愀 瀀椀瘀漀琀愀氀 爀漀氀攀 椀渀 琀栀攀 椀渀搀甀挀琀椀漀渀 漀昀 攀昀昀攀挀琀漀爀 吀 挀攀氀氀猀 琀栀愀琀 挀漀渀琀爀漀氀 倀戀 椀渀昀攀挀琀椀漀渀⸀ 䤀渀 琀栀椀猀 猀琀甀搀礀Ⰰ 眀攀 愀渀愀氀礀稀攀搀 琀栀攀 爀漀氀攀 琀栀愀琀 䐀䌀 瀀氀愀礀攀搀 椀渀 洀漀搀甀氀愀琀椀渀最 倀戀 椀渀昀攀挀琀椀漀渀⸀  We observed an increased expression of CCR7 and CD103 on lung DC after infection, as well as MHC-II. After Pb infection, bone marrow-derived DC as well lung DC, migrate to lymph nodes. Migration of lung DC could represent an important mechanism of pathogenesis during PCM infection. In resume our data showing that Pb induces migration of DC. Furthermore, we demonstrated that bone marrow-derived DC stimulated by Pb migrate to the lymph nodes and activate a T helper (Th) response. Elucidating the mechanisms of regulation and coordination of DC migration by pathogen-derived signals requires more study. An understanding of the signals that regulate DC migration could allow for the development of methods to induce efficient T-cell activation that would aid in the control of PCM infection.਀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀䬀攀礀眀漀爀搀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀䐀攀渀搀爀椀琀椀挀 挀攀氀氀Ⰰ 氀甀渀最Ⰰ 吀 挀攀氀氀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀