XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:95-1

Poster (Painel)

95-1	Paracoccidioides.brasiliensis proteomic analysis during cooper deprivation
Authors:	Laura Maria Barbosa Gonçalves (UFG - Universidade Federal de GoiásUFG - Universidade Federal de Goiás) ; Alexandre Melo Bailão (UFG - Universidade Federal de Goiás) ; Clayton Luiz Borges (UFG - Universidade Federal de Goiás) ; Mike S.winters (UCCM - University of Cincinnati College of Medicine) ; Célia Maria de Almeida Soares (UFG - Universidade Federal de Goiás)

Abstract

The thermally dimorphic fungus Paracoccidioides brasiliensis is the ethiological agent of paracoccidioidomycosis, a common systemic mycosis in Latin America. To experience a successful colonization of the host, microorganisms must obtain essential nutrients for growth. Copper is one of those important nutrients for the establishment of infection, and is required as a cofactor for a variety of enzymes in biological processes essential for cellular maintenance. This work aims to identify proteins regulated by copper depletion. The fungus Pb 01 isolate (ATCCMYA-826) was incubated in liquid minimal medium in the presence or absence (added of BCS: batocuproinadisulfonato sodium) of copper for 24 h and 48 h of copper deprivation. The proteins were fractionated by two-dimensional electrophoresis and image analysis allowed the identification of 82 spots differentially expressed. The differentially expressed proteins were subjected to tryptic digestion and peptides were analyzed by mass spectrometry. The identification of proteins was performed using Mascot software http://www.matrixscience.com. Preliminary results have identified 56 proteins differentially expressed upon copper deficiency; 21 proteins were induced in the time of 24 h and 6 in time 48 h. Proteins with reduced expression were 17 and 12, respectively after 24 h and 48 h . Copper regulated proteins were involved in energy, metabolism, cell rescue and virulence, protein synthesis, cell cycle and protein fate. Recent studies indicate that copper modulates critical determinants of virulence of fungal pathogens. Evidence from genomic studies has shown that copper modulates transcriptional changes not only of genes directly associated with copper metabolism but also of genes that participate in general physiologic processes. We observed the increased expression of glycolytic enzymes during copper deprivation, while enzymes related to the tricarboxylic acid cycle and electron transport chain were down regulated. Data suggest a remodeling of the fungal metabolism during copper deficiency. Additional analysis are under progress.਀ Financial support: CNPq, FINEP, FAPEG.

Keyword: Paracoccidioidomycosis, Proteomic Analysis, Copper deprivation

਀�