XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:84-1

Poster (Painel)

84-1

Antifungal activity of Altenusin isolated from the tropical endophytic fungus Alternaria sp. against clinical fungal strains of Paracoccidioides brasiliensis

Authors:

Susana Johann (UFMG - Universidade Federal de Minas GeraisFIOCRUZ - Fundação Oswaldo Cruz) ; Luiz Henrique Rosa (UFMG - Universidade Federal de Minas Gerais) ; Carlos Augusto Rosa (UFMG - Universidade Federal de Minas Gerais) ; Pilar Perez (US - Universidad de Salamanca) ; Patrícia Silva Cisalpino (UFMG - Universidade Federal de Minas Gerais) ; Betânia Barros Cota (FIOCRUZ - Fundação Oswaldo Cruz)

Abstract

The pathogenic fungus Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis (PCM), a human systemic mycosis for which the portal of entry of the fungus is via inhalation of airborne propagules into the respiratory tract. Although geographically confined,paracoccidioidomycosis constitutes one of the most prevalent deep mycoses in Central and Southern America region of the world. Antifungal chemotherapy is routinely used in cases of PCM, with initial treatment lasting from 2 to 6 months and involving sulfonamides, amphotericin B, or azoles. This extended period of treatment is necessary due to the significant frequency of relapsing disease.਀ 䄀氀琀攀渀甀猀椀渀椀猀愀戀椀瀀栀攀渀礀氀搀攀爀椀瘀愀琀椀瘀攀椀猀漀氀愀琀攀搀昀爀漀洀搀椀昀昀攀爀攀渀琀猀瀀攀挀椀攀猀漀昀洀椀挀爀漀漀爀最愀渀椀猀洀猀Ⰰ 眀栀椀挀栀猀栀漀眀猀搀椀昀昀攀爀攀渀琀戀椀漀氀漀最椀挀愀氀瀀爀漀瀀攀爀琀椀攀猀⸀ 䤀渀琀栀攀瀀爀攀猀攀渀琀眀漀爀欀Ⰰ 眀攀爀攀瀀漀爀琀愀渀琀椀昀甀渀最愀氀愀挀琀椀瘀椀琀礀漀昀愀氀琀攀渀甀猀椀渀椀猀漀氀愀琀攀搀昀爀漀洀攀渀搀漀瀀栀礀琀椀挀昀甀渀最甀猀㰀椀㸀䄀氀琀攀爀渀愀爀椀愀㰀⼀椀㸀猀瀀⸀ 唀䘀䴀䜀䌀䈀㔀㔀愀最愀椀渀猀琀挀氀椀渀椀挀愀氀椀猀漀氀愀琀攀猀漀昀㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀愀猀眀攀氀氀愀猀椀琀猀愀挀琀椀漀渀漀渀挀攀氀氀猀眀愀氀氀猀漀昀㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀愀渀搀琀栀攀洀漀搀攀氀礀攀愀猀琀㰀椀㸀匀挀栀椀稀漀猀愀挀挀栀愀爀漀洀礀挀攀猀瀀漀洀戀攀㰀⼀椀㸀⸀ The in vitro antifungal activities of this compound were evaluated using the broth microdilution method against eleven clinical strains of P. brasiliensis and one of S. pombe. The altenusin presented strong activity against P. brasiliensis with MIC range between 1.9 to 30µg/mL and minor activity against S. pombe with MIC value of 62.5µg/ml. The effects of the altenusin on the fungal cell wall were estimated using the sorbitol assay on cells of S. pombe. ਀伀甀爀爀攀猀甀氀琀猀搀攀洀漀渀猀琀爀愀琀攀搀琀栀愀琀眀栀攀渀琀栀攀洀攀搀椀甀洀猀甀瀀瀀氀攀洀攀渀琀攀搀眀椀琀栀猀漀爀戀椀琀漀氀眀愀猀琀爀攀愀琀攀搀眀椀琀栀愀氀琀攀渀甀猀椀渀Ⰰ 琀栀攀䴀䤀䌀瘀愀氀甀攀猀眀攀爀攀栀椀最栀攀爀昀漀爀㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀 ⠀倀戀㄀㠀⤀ ⠀䴀䤀䌀瘀愀氀甀攀漀昀㘀㈀⸀㔀딀最⼀洀氀⤀ 愀渀搀昀漀爀匀⸀ 瀀漀洀戀攀 ⠀䴀䤀䌀瘀愀氀甀攀漀昀㄀㈀㔀 딀最⼀洀氀⤀⸀ 䤀渀昀氀甀漀爀攀猀挀攀渀挀攀洀椀挀爀漀猀挀漀瀀礀Ⰰ 甀猀椀渀最䌀愀氀挀漀昀氀甀漀爀眀栀椀琀攀愀猀猀琀愀椀渀Ⰰ 琀栀攀挀攀氀氀猀漀昀琀栀攀㰀椀㸀匀⸀ 瀀漀洀戀攀㰀⼀椀㸀琀爀攀愀琀攀搀眀椀琀栀愀氀琀攀渀甀猀椀渀眀攀爀攀洀漀爀攀爀漀甀渀搀攀搀琀栀愀渀甀渀琀爀攀愀琀攀搀挀攀氀氀⸀ 吀栀攀猀攀昀椀渀搀椀渀最猀猀甀最最攀猀琀琀栀愀琀愀氀琀攀渀甀猀椀渀眀漀甀氀搀愀挀琀琀栀爀漀甀最栀琀栀攀椀渀栀椀戀椀琀椀漀渀漀昀挀攀氀氀眀愀氀氀猀礀渀琀栀攀猀椀猀漀爀愀猀猀攀洀戀氀礀椀渀戀漀琀栀㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀愀渀搀㰀椀㸀匀⸀ 瀀漀洀戀攀㰀⼀椀㸀愀渀搀爀攀瀀爀攀猀攀渀琀愀渀椀渀琀攀爀攀猀琀椀渀最瀀爀漀琀漀琀礀瀀攀洀漀氀攀挀甀氀攀昀漀爀搀攀瘀攀氀漀瀀洀攀渀琀漀昀愀渀琀椀昀甀渀最愀氀搀爀甀最猀⸀

Keyword: Altenusin, Cell wall, Paracoccidiodomycosis

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