XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:81-1

Oral / Poster

81-1

Immunomodulating Activity of Antifungal Drug: Itraconazole

Authors:

Tonny Williams Naranjo Preciado (CIB - Corporacion para Investigaciones BiologicasUPB - Universidad Pontificia Bolivariana) ; Damaris Elena Lopera (CIB - Corporacion para Investigaciones Biologicas) ; Angela Restrepo Moreno (CIB - Corporacion para Investigaciones Biologicas) ; Luz Elena Cano Restrepo (CIB - Corporacion para Investigaciones BiologicasUDEA - Universidad de AntioquiaUPB - Universidad Pontificia Bolivariana) ; Andres Felipe Zuluaga (UDEA - Universidad de Antioquia)

Abstract

Treatment of most superficial and systemic fungal infections is focused in a limited number of antifungal drugs, namely, polyenes, echinocandins and azolic derivates, among them, itraconazole (ITC), a medication frequently prescribed and considered the treatment of choice for most of the endemic mycoses. Although its antifungal activity has been clearly established, its immunomodulatory activity is not well known. The present study investigated ITC’s effects as modulator compound on twenty-eight molecules endowed with immunological activity, including anti- and pro-inflammatory cytokines, chemokines and growth factors, taking into consideration their lung concentrations in healthy mice. Two experimental groups of 20 BALB/c male mice each were used as follow: (a) control group, PBS treated mice; (b) ITC treated mice (1 mg/day). Mice were treated daily via gavage for 8 weeks and sacrificed at 0, 4, 8 and 12 weeks; their lungs were dissected, macerates and the supernatants used to measure pulmonary cytokine levels by the Bio-Plex® system. Further, lungs from five ITC treated mice were used for histopathological analysis. The statistical analyses were performed by student t-test assuming a significant difference when p <0.05.਀䤀渀挀漀洀瀀愀爀椀猀漀渀眀椀琀栀琀栀攀挀漀渀琀爀漀氀最爀漀甀瀀Ⰰ 䤀吀䌀ⴀ琀爀攀愀琀攀搀洀椀挀攀猀栀漀眀攀搀猀椀最渀椀昀椀挀愀渀琀挀栀愀渀最攀猀椀渀瀀甀氀洀漀渀愀爀礀氀攀瘀攀氀猀椀渀愀氀洀漀猀琀㔀　─ 漀昀琀栀攀洀漀氀攀挀甀氀攀猀琀攀猀琀攀搀眀椀琀栀猀漀洀攀漀昀琀栀攀洀攀砀瀀攀爀椀洀攀渀琀椀渀最猀瀀攀挀椀昀椀挀挀栀愀渀最攀猀愀琀琀栀攀戀攀最椀渀漀爀愀琀琀栀攀攀渀搀漀昀琀爀攀愀琀洀攀渀琀 ⠀䤀䰀ⴀ㄀☀⌀㤀㐀㔀㬀Ⰰ 䤀䰀ⴀ㄀☀⌀㤀㐀㘀㬀Ⰰ 䤀䰀ⴀ㈀Ⰰ 䴀䤀倀ⴀ㄀☀⌀㤀㐀㔀㬀Ⰰ 䴀䤀倀ⴀ㈀Ⰰ 䰀䤀䘀Ⰰ 嘀䔀䜀䘀⤀Ⰰ 漀琀栀攀爀猀攀砀栀椀戀椀琀攀搀愀氀琀攀爀愀琀椀漀渀猀搀甀爀椀渀最琀栀攀琀爀攀愀琀洀攀渀琀愀渀搀攀瘀攀渀㐀眀攀攀欀猀愀昀琀攀爀琀栀攀攀渀搀 ⠀䤀䰀ⴀ㐀Ⰰ 䤀䰀ⴀ㄀　Ⰰ 䤀䰀ⴀ㄀㈀瀀㜀　Ⰰ 䤀䰀ⴀ㄀㌀Ⰰ 䤀䰀ⴀ㄀㔀Ⰰ 吀一䘀ⴀ☀⌀㤀㐀㔀㬀Ⰰ 䔀漀琀愀砀椀渀⤀⸀ 䐀攀猀瀀椀琀攀琀栀攀昀愀挀琀琀栀愀琀琀栀攀栀椀猀琀漀瀀愀琀栀漀氀漀最椀挀愀氀愀渀愀氀礀猀椀猀搀椀搀渀漀琀爀攀瘀攀愀氀挀栀愀渀最攀猀椀渀琀栀攀氀甀渀最猀挀攀氀氀猀椀渀昀椀氀琀爀愀琀攀猀Ⰰ 琀栀椀猀猀琀甀搀礀搀攀洀漀渀猀琀爀愀琀攀搀琀栀愀琀䤀吀䌀攀砀攀爀琀猀愀洀愀爀欀攀搀椀洀洀甀渀漀洀漀搀甀氀愀琀漀爀礀攀昀昀攀挀琀愀琀瀀甀氀洀漀渀愀爀礀氀攀瘀攀氀椀渀栀攀愀氀琀栀礀洀椀挀攀⸀

Keyword: Itraconazol, Immunomodulation, Cytokines

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