Abstract

Introduction: Any infectious process starts with the initial contact between pathogen and host. It is known that certain pathogens interact with the host's coagulation system. We had previously demonstrated that Paracoccidioides brasiliensis conidia interact with plasma proteins including fibronectin and fibrinogen, the latter considered a component of the coagulation system. In the present study, we evaluated the in vitro aggregation capacity of P. brasiliensis conidia with plasma proteins and various molecules connected with the coagulation system.਀䴀攀琀栀漀搀漀氀漀最礀㨀 圀攀 愀猀猀攀猀猀攀搀 戀礀 洀攀愀渀猀 漀昀 猀琀愀渀搀愀爀搀椀稀攀搀 洀攀琀栀漀搀猀Ⰰ 愀最最爀攀最愀琀椀漀渀 漀昀 㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀 挀漀渀椀搀椀愀 漀渀挀攀 椀渀挀甀戀愀琀攀搀 眀椀琀栀 猀攀爀甀洀 漀爀 栀甀洀愀渀 瀀氀愀猀洀愀 椀渀 瀀爀攀猀攀渀挀攀 漀爀 愀戀猀攀渀挀攀 漀昀 愀渀琀椀挀漀愀最甀氀愀渀琀猀Ⰰ 攀砀琀爀愀挀攀氀氀甀氀愀爀 洀愀琀爀椀砀 瀀爀漀琀攀椀渀猀 ⠀䔀䌀䴀瀀⤀Ⰰ 洀攀琀愀戀漀氀椀挀ⴀ 愀渀搀 瀀爀漀琀攀椀渀ⴀ椀渀栀椀戀椀琀漀爀猀Ⰰ 洀漀渀漀 猀愀挀挀栀愀爀椀搀攀猀Ⰰ 愀渀搀 漀琀栀攀爀 挀漀洀瀀漀甀渀搀猀⸀ 䄀搀搀椀琀椀漀渀愀氀氀礀Ⰰ 瀀爀漀琀栀爀漀洀戀椀渀 愀渀搀 瀀愀爀琀椀愀氀 琀栀爀漀洀戀漀瀀氀愀猀琀椀渀 琀椀洀攀猀 眀攀爀攀 搀攀琀攀爀洀椀渀攀搀 愀昀琀攀爀 椀渀琀攀爀愀挀琀椀漀渀 漀昀 㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀 挀漀渀椀搀椀愀 眀椀琀栀 栀甀洀愀渀 瀀氀愀猀洀愀⸀ Results: ECMp, monosaccharides and human plasma significantly induced P. brasiliensis conidial aggregation; on the contrary, metabolic- and protein- inhibitors, as well as anticoagulants decreased this aggregation process. When a monoclonal antibody addressed against an adhesin located on the cell wall of P. brasiliensis, that binds ECMp, a slightly decrease in the aggregation process was observed. The extrinsic pathway of coagulation was not affected by the interaction between P. brasiliensis conidia and plasma proteins while the intrinsic pathway was markedly altered. ਀䌀漀渀挀氀甀猀椀漀渀㨀 吀栀攀猀攀 爀攀猀甀氀琀猀 椀渀搀椀挀愀琀攀 琀栀愀琀 㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀 挀漀渀椀搀椀愀 椀渀琀攀爀愀挀琀 眀椀琀栀 瀀爀漀琀攀椀渀猀 椀渀瘀漀氀瘀攀搀 椀渀 琀栀攀 挀漀愀最甀氀愀琀椀漀渀 猀礀猀琀攀洀⸀ 吀栀椀猀 椀渀琀攀爀愀挀琀椀漀渀 洀愀礀 瀀氀愀礀 愀渀 椀洀瀀漀爀琀愀渀琀 爀漀氀攀 椀渀 琀栀攀 椀渀椀琀椀愀氀 椀渀昀氀愀洀洀愀琀漀爀礀 爀攀猀瀀漀渀猀攀Ⰰ 愀猀 眀攀氀氀 愀猀 椀渀 瀀爀漀最爀攀猀猀椀漀渀 漀昀 琀栀攀 昀甀渀最愀氀 搀椀猀攀愀猀攀 愀挀挀漀洀瀀愀渀椀攀搀 戀礀 㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀 搀椀猀猀攀洀椀渀愀琀椀漀渀⸀ Financial support: support of this study was provided by the Fundación para la Promoción de la Investigación y la Tecnología del Banco de la República, Grant No. 2050. ਀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀䬀攀礀眀漀爀搀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀倀愀爀愀挀漀挀挀椀搀椀漀椀搀攀猀 戀爀愀猀椀氀椀攀渀猀椀猀Ⰰ 䌀漀愀最甀氀愀琀椀漀渀 猀礀猀琀攀洀Ⰰ 䘀椀戀爀椀渀漀最攀渀Ⰰ 倀氀愀猀洀愀 瀀爀漀琀攀椀渀猀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀