Abstract

The transition ability of P. brasiliensis and the capacity to grow as a yeast form in the host tissues are essential for the development of the disease and later dissemination to other tissues. There are many proteins involved in this switch, among them are the heat shock proteins (Hsp), which have been postulated to play an important role in the adaptation of the cells to environment conditions. Hsp90 is one of the proteins that enable this phenotypic diversity in the fungus, in response to different stress conditions, such as changes in temperature, low pH or cells exposure to oxidative stress.਀唀猀椀渀最 愀渀琀椀猀攀渀猀攀 刀一䄀Ⰰ 愀 琀攀挀栀渀椀焀甀攀 漀昀 瀀漀猀琀ⴀ琀爀愀渀猀挀爀椀瀀琀椀漀渀愀氀 猀椀氀攀渀挀椀渀最Ⰰ 眀攀 搀攀瘀攀氀漀瀀攀搀 愀 猀琀爀愀椀渀 眀椀琀栀 搀漀眀渀 爀攀最甀氀愀琀椀漀渀 漀昀 琀栀攀 最攀渀攀 ⠀倀戀䠀猀瀀㤀　ⴀ愀刀一䄀⤀⸀ 䰀愀琀攀爀Ⰰ 眀攀 挀栀愀氀氀攀渀最攀搀 琀栀攀 倀⸀  戀爀愀猀椀氀椀攀渀猀椀猀 眀椀氀搀 琀礀瀀攀 ⠀倀戀圀琀⤀ 愀渀搀 倀戀䠀猀瀀㤀　ⴀ愀刀一䄀 猀琀爀愀椀渀猀 眀椀琀栀 挀漀渀搀椀琀椀漀渀猀 琀栀愀琀 倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀 眀椀氀氀 昀愀挀攀 搀甀爀椀渀最 昀甀渀最愀氀 洀攀琀愀戀漀氀椀猀洀 愀渀搀 栀漀猀琀 椀渀琀攀爀愀挀琀椀漀渀猀Ⰰ 猀甀挀栀 愀猀 愀挀椀搀 攀渀瘀椀爀漀渀洀攀渀琀猀 愀渀搀 攀砀瀀漀猀甀爀攀 琀漀 爀攀愀挀琀椀瘀攀 漀砀礀最攀渀 猀瀀攀挀椀攀猀 ⠀刀伀匀⤀Ⰰ 眀栀椀挀栀 漀挀挀甀爀 搀甀爀椀渀最 椀渀琀攀爀愀挀琀椀漀渀猀 眀椀琀栀 洀愀挀爀漀瀀栀愀最攀猀⸀ Acid environments induced an increased expression of hsp90 in PbWt, with a small drop in cell viability, whereas the low gene expression observed in PbHsp90-aRNA yeast resulted in a large decrease in cell viability, both in neutral and acid pH conditions of growth although the decrease in viability was more evident at acid pH. In a similar manner, the interaction of Pb yeast with macrophage cells induced the expression of the hsp90 gene in PbWt, with no alterations in cell viability, while low expression levels of this gene were observed during the interaction of PbHsp90-aRNA with macrophage cells with a decrease of cell viability in these cells.਀吀栀攀 搀漀眀渀 爀攀最甀氀愀琀椀漀渀 漀昀 栀猀瀀㤀　 挀氀攀愀爀氀礀 搀椀洀椀渀椀猀栀攀猀 琀栀攀 瘀椀愀戀椀氀椀琀礀 漀昀 琀栀攀 挀攀氀氀猀Ⰰ 愀渀搀 爀攀猀甀氀琀猀 椀渀 愀 栀椀最栀攀爀 猀攀渀猀椀琀椀瘀椀琀礀 漀昀 琀栀攀 礀攀愀猀琀 琀漀 愀挀椀搀 瀀䠀 愀渀搀 刀伀匀 琀栀愀渀 琀漀 栀礀搀爀漀最攀渀 瀀攀爀漀砀椀搀攀㬀 攀瘀攀渀 椀渀 琀栀攀 愀戀猀攀渀挀攀 漀昀 瀀栀礀猀椀漀氀漀最椀挀愀氀 猀琀爀攀猀猀 琀栀攀 挀攀氀氀猀 猀栀漀眀攀搀 愀 搀攀挀爀攀愀猀攀 椀渀 挀攀氀氀 瘀椀愀戀椀氀椀琀礀⸀ 吀栀攀猀攀 昀椀渀搀椀渀最猀 猀甀瀀瀀漀爀琀 琀栀攀 爀漀氀攀 漀昀 䠀匀倀㤀　 搀甀爀椀渀最 琀栀攀 挀攀氀氀가∠猡 愀搀愀瀀琀愀琀椀漀渀 琀漀 栀漀猀琀椀氀攀 攀渀瘀椀爀漀渀洀攀渀琀猀 椀渀 漀爀搀攀爀 琀漀 攀渀猀甀爀攀 琀栀攀 猀甀爀瘀椀瘀愀氀 漀昀 琀栀攀 昀甀渀最甀猀 搀甀爀椀渀最 栀漀猀琀 椀渀琀攀爀愀挀琀椀漀渀⸀ We thank to Universidad de Antioquia and the Cellular and Molecular Biology Unit, Corporacion para Investigaciones Biológicas (CIB) for financial support.਀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀䬀攀礀眀漀爀搀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀倀愀爀愀挀漀挀挀椀搀椀漀椀搀攀猀 戀爀愀猀椀氀椀攀渀猀椀猀Ⰰ 䜀攀渀攀 猀椀氀攀渀挀椀渀最Ⰰ 䠀匀倀㤀　Ⰰ 䌀攀氀氀 瘀椀愀戀椀氀椀琀礀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀