XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:73-11

Poster (Painel)

73-11

Histological evaluation of experimental paracoccidioidomicosis wistar rats after total splenectomy and immunosuppression by Cyclosporin A

Authors:

Samia Khalil Biazin (UNIOESTE - Universidade Estadual do Oeste do Paraná) ; Michele Ana Flores Chaves (UNIOESTE - Universidade Estadual do Oeste do Paraná) ; Emerson Machado (PREVENÇÃO & DIAGNOSE - laboratório Prevenção e Diagnose) ; Fabio Negretto (UNIOESTE - Universidade Estadual do Oeste do Paraná) ; Rinaldo Ferreita Gandra (UNIOESTE - Universidade Estadual do Oeste do Paraná) ; Eduardo Alexandre Loth (UNIOESTE - Universidade Estadual do Oeste do Paraná)

Abstract

Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensisI (CP) occurs primarily through the upper airway and the most common in the lungs. From the lungs, the fungus can spread throughout the body. The study aimed to evaluate the histological changes in experimental model of PCM in animals splenectomized and immunosuppressed with Cyclosporine A. METHODS: Animals Wistar rats, male, with 2 months of life. Divided into four groups: Group Splenectomy (n = 8) underwent laparotomy for splenectomy. The laparotomy group underwent laparotomy without splenectomy. The group Cyclosporin A was subjected to a daily dose of human Ciclosporina A 10 mg/kg by gavage during the experiment. After 7 days of surgery and the beginning of the use of cyclosporin A, the animals were subjected to intraperitoneal inoculation of Pb18 suspended at a concentration of 106. The control group underwent intraperitoneal inoculation of PBS. 30 days after inoculation, the animals were killed to collect samples of lung and liver for processing and histological analysis and morphometry. RESULTS Histological examination of the splenectomy group showed pulmonary infection in 100% of animals with the presence of intense granulomatous inflammatory process with granulomas organized and unorganized. Only 5 (63%) animals in this group had concomitant infection in the liver, which was mild inflammation. Cyclosporine in the group observed that 100% of livers examined showed granulomatous inflammation intense. In lung biopsy specimens, 4 (50%) animals showed moderate granulomatous inflammation and Pb intestício. In the laparotomy group, the lung was the organ primarily affected. However, 4 (50%) of the animals, histological changes in the liver. The average area of granulomas found in the livers and lungs of the animals was 10,675 and μm2 μm2 8951, 8464 and 10,497 μm2, 8. 320μm2 and 10,340 μm2 Ciclosporinta groups, laparotomy and splenectomy, respectively. CONCLUSION: Pb infection in laparotomy and splenectomy groups followed similar patterns, whose organ most affected was the lung. In Group Cyclosporine, the organ most affected was the liver.਀

Keyword: paracoccidioidomycosis, immunosuppression, Cyclosporin A, splenectomy, Paracoccidioides brasiliensis

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