XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:73-10

Poster (Painel)

73-10	Evaluation of radiation and edema in experimental model of arthritis fungal with Paracoccidioides brasiliensis
Authors:	Eduardo Alexandre Loth (UNIOESTE - universidade Estadual do Oeste do Paraná) ; José Roberto Leonel Ferreira (UNIOESTE - universidade Estadual do Oeste do Paraná) ; Rinaldo Ferreira Gandra (UNIOESTE - universidade Estadual do Oeste do Paraná) ; Samia Khalil Biazin (UNIOESTE - universidade Estadual do Oeste do Paraná) ; Marcelo Fabiando de Franco (UNIFESP - Universidade Federal de São Paulo)

Abstract

INTRODUCTION Paracoccidioidomycosis (PCM) is a disease caused by Paracoccidioides brasiliensis (Pb), being more prevalent in Brazil, is the major cause of deaths from systemic mycosis, primarily affects men aged 30 to 50 productive years, the disease has usually pulmonary focus of this website and spread to other systems. Including joint system. The study aimed to evaluate the radiological changes of the experimental model of arthritis by fungal P. brasiliensis. MATERIALS AND METHODS: We used 32 Wistar rats, 4 groups, males, and 2 months old. Groups GE/15 GE/45 and received one dose of 100 μl of phosphate buffered saline (PBS) containing 105 Pb yeasts, viable, suspended in the same knee. The groups and GCA/15 GCA/45 (negative control) received a single dose of 100 μl of PBS satisfaction 105 Pb yeasts, viable in suspension. The animals were sacrificed 15 and 45 days after the date of inoculation. Before sacrificing the animals from groups and GE/15 GCA/15 (15 days after inoculation) and the groups and GE/45 GCA/45 (45 days after inoculation), were reassessed by the above techniques. The values obtained were qualitatively analyzed by simple descriptive statistics. Numerical variables resulting from the measurement of edema were analyzed by statistical analysis, the 5% significance. RESULTS AND DISCUSSION: Results indicate that the diameter of the joints infused increased 17.12% and 0.37% in GE/15 GCA/15 in the final evaluation. Observed increase in joint diameter GE/45 of 14.39% and 1.30% in GCA/45. The radiological examination in 100% of animals and GE/15 GE/45 at the end of the study showed increased bone density, joint space narrowing, and irregularity on the articular surfaces, deformities of the epiphyseal lines, flattened bone. In the other groups, the radiographs showed integrity in the structures of the joints from beginning to end of the experiment. CONCLUSIONS: We can conclude that the method was able to induce septic arthritis in the knees of rats causing swelling and radiographic changes.

Keyword: Paracoccidioidomycosis, Paracoccidioides brasiliensis, experimental arthritis, radiology, fungal arthritis

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