XI International Meeting on Paracoccidioidomycosis

XI International Meeting on Paracoccidioidomycosis

Resume:57-1

Oral / Poster

57-1	In vitro evaluation of the susceptibility of Paracoccidioides brasiliensis to amphotericin B e azole compounds
Authors:	Adriana de Oliveira Afonso (FMRP-USP - Faculty of Medicine of Ribeirão Preto University of S. Paulo) ; Lúcia Helena Vitali (FMRP-USP - Faculty of Medicine of Ribeirão Preto University of S. Paulo) ; Claudia Maria Leite Maffei (FMRP-USP - Faculty of Medicine of Ribeirão Preto University of S. Paulo) ; Roberto Martinez (FMRP-USP - Faculty of Medicine of Ribeirão Preto University of S. Paulo)

Abstract

INTRODUCTION - In vitro susceptibility tests for dimorphic fungi, especially Paracoccidioides brasiliensis, are scarce and the results are not always concordant. Assessing the sensitivity of P. brasiliensis to antifungal agents can be useful for the selection of drugs for empirical treatment and for individualized therapy in problematic cases.਀䴀䔀吀䠀伀䐀伀䰀伀䜀夀 ⴀ 圀攀甀猀攀搀琀栀攀戀爀漀琀栀洀愀挀爀漀搀椀氀甀琀椀漀渀琀攀猀琀搀攀猀挀爀椀戀攀搀椀渀琀栀攀䴀㈀㜀ⴀ䄀㈀搀漀挀甀洀攀渀琀漀昀琀栀攀䌀䰀匀䤀⸀ 圀攀甀猀攀搀㔀愀渀琀椀昀甀渀最愀氀搀爀甀最猀椀渀猀攀爀椀愀氀搀椀氀甀琀椀漀渀猀㨀愀洀瀀栀漀琀攀爀椀挀椀渀䈀愀渀搀椀琀爀愀挀漀渀愀稀漀氀攀 ⠀　⸀　㄀㔀㘀琀漀㄀㘀 딀最⼀洀䰀⤀Ⰰ 欀攀琀漀挀漀渀愀稀漀氀攀 ⠀　⸀　　㜀㠀琀漀㄀㘀 딀最⼀洀䰀⤀Ⰰ 昀氀甀挀漀渀愀稀漀氀攀Ⰰ 愀渀搀瘀漀爀椀挀漀渀愀稀漀氀攀 ⠀　⸀　㘀㈀㔀琀漀㘀㐀 딀最⼀洀䰀⤀⸀ 圀攀攀洀瀀氀漀礀攀搀㈀㌀挀氀椀渀椀挀愀氀椀猀漀氀愀琀攀猀漀昀㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀Ⰰ 礀攀愀猀琀ⴀ氀椀欀攀瀀栀愀猀攀⸀ 吀栀攀昀甀渀最愀氀椀渀漀挀甀氀甀洀眀愀猀瀀爀攀瀀愀爀攀搀椀渀刀倀䴀䤀㄀㘀㐀　猀甀瀀瀀氀攀洀攀渀琀攀搀漀爀渀漀琀眀椀琀栀㈀─ 最氀甀挀漀猀攀愀渀搀愀搀樀甀猀琀攀搀琀漀㄀⸀　堀㄀　㐀䘀甀渀最愀氀唀渀椀琀猀⼀洀䰀 ⠀䘀唀⼀洀䰀⤀ 昀漀爀愀洀瀀栀漀琀攀爀椀挀椀渀䈀愀渀搀琀漀㄀⸀　堀㄀　㔀䘀唀⼀洀氀昀漀爀琀栀攀琀攀猀琀猀眀椀琀栀琀栀攀愀稀漀氀攀愀最攀渀琀猀⸀ 吀栀攀洀椀渀椀洀甀洀椀渀栀椀戀椀琀漀爀礀挀漀渀挀攀渀琀爀愀琀椀漀渀 ⠀䴀䤀䌀⤀ 眀愀猀搀攀琀攀爀洀椀渀攀搀愀昀琀攀爀㜀搀愀礀猀漀昀椀渀挀甀戀愀琀椀漀渀Ⰰ 戀攀椀渀最挀漀渀猀椀搀攀爀攀搀愀猀琀栀攀氀漀眀攀猀琀搀爀甀最挀漀渀挀攀渀琀爀愀琀椀漀渀琀栀愀琀椀渀栀椀戀椀琀攀搀㄀　　─ 愀渀搀㠀　─ 漀昀琀攀猀琀琀甀戀攀最爀漀眀琀栀昀漀爀愀洀瀀栀漀琀攀爀椀挀椀渀䈀愀渀搀琀栀攀愀稀漀氀攀挀漀洀瀀漀甀渀搀猀Ⰰ 爀攀猀瀀攀挀琀椀瘀攀氀礀Ⰰ 挀漀洀瀀愀爀攀搀琀漀挀漀渀琀爀漀氀琀甀戀攀猀⸀ RESULTS - The MICs ranged from 0.0312 to 0.25 µg/ml for amphotericin B, from < 0.0078 to 0.0625 µg/ml for ketoconazole, from < 0.0156 to 0.25 µg/ml for itraconazole, from < 0.0625 to 0.25 µg/ml for fluconazole, and from < 0.0625 to 0.125 µg/ml for voriconazole, with respective MIC90 values of 0.25 µg/ml, 0.0625 µg/ml, 0.0625 µg/ml, 0.25 µg/ml, and 0.125 µg/ml.਀䌀伀一䌀䰀唀匀䤀伀一 ⴀ 吀栀攀戀氀漀漀搀氀攀瘀攀氀猀愀挀栀椀攀瘀攀搀眀椀琀栀琀栀攀甀猀甀愀氀琀栀攀爀愀瀀攀甀琀椀挀搀漀猀攀猀漀昀琀栀攀愀渀琀椀昀甀渀最愀氀愀最攀渀琀猀琀攀猀琀攀搀愀爀攀洀甀挀栀栀椀最栀攀爀琀栀愀渀琀栀攀䴀䤀䌀猀搀攀琀攀挀琀攀搀㰀椀㸀椀渀瘀椀琀爀漀㰀⼀椀㸀椀渀琀栀攀瀀爀攀猀攀渀琀猀琀甀搀礀⸀ 䤀渀琀栀攀瀀爀攀猀攀渀琀猀琀甀搀礀Ⰰ 愀猀愀氀猀漀爀攀瀀漀爀琀攀搀椀渀琀栀攀氀椀琀攀爀愀琀甀爀攀Ⰰ 氀漀眀䴀䤀䌀瘀愀氀甀攀猀眀攀爀攀漀戀猀攀爀瘀攀搀昀漀爀欀攀琀漀挀漀渀愀稀漀氀攀愀渀搀椀琀爀愀挀漀渀愀稀漀氀攀⸀ 吀栀攀䴀䤀䌀猀昀漀爀昀氀甀挀漀渀愀稀漀氀攀愀爀攀甀猀甀愀氀氀礀栀椀最栀攀爀琀栀愀渀琀栀漀猀攀漀戀琀愀椀渀攀搀昀漀爀欀攀琀漀挀漀渀愀稀漀氀攀愀渀搀椀琀爀愀挀漀渀愀稀漀氀攀⸀ 䘀漀爀㰀椀㸀倀⸀ 戀爀愀猀椀氀椀攀渀猀椀猀㰀⼀椀㸀Ⰰ 琀栀攀猀䴀䤀䌀猀昀漀爀瘀漀爀椀挀漀渀愀稀漀氀攀愀渀搀椀琀爀愀挀漀渀愀稀漀氀攀眀攀爀攀猀椀洀椀氀愀爀琀漀愀渀搀氀漀眀攀爀琀栀愀渀琀栀攀䴀䤀䌀昀漀爀昀氀甀挀漀渀愀稀漀氀攀Ⰰ 愀猀愀氀猀漀爀攀瀀漀爀琀攀搀椀渀琀栀攀氀椀琀攀爀愀琀甀爀攀⸀ SUPPORT - Research and Technological Development Unit (UPDT), National STD/AIDS Program.

Keyword: Paracoccidioides brasiliensis, Susceptibility tests, Minimum inhibitory concentration, Amphotericin B, Azole compounds

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