XI International Meeting on Paracoccidioidomycosis
Resume:45-6


Poster (Painel)
45-6ANALYSIS OF THE INTRINSIC PARAMETERS OF DOUBLE IMMUNODIFFUSION AND IMMUNOBLOTTING ASSAYS FOR THE PARACOCCIDIOIDOMYCOSIS DIAGNOSIS.
Authors:Angela Noronha Passos (IAL - Instituto Adolfo LutzPG-CCD-SES-SP - Programa de Pos Graduacao em Ciencias (CCD-SES-SP)) ; Valdelene Sayuri Kohara (IAL - Instituto Adolfo Lutz) ; Talisia Collachiti Moreto (FMB - Faculdade de Medicina-Unesp Botucatu) ; Rinaldo Poncio Mendes (FMB - Faculdade de Medicina-Unesp Botucatu) ; Camila Mika Kamikawa (IAL - Instituto Adolfo Lutz) ; Adriana Pardini Vicentini (IAL - Instituto Adolfo Lutz)

Abstract

Search for anti-Paracoccidioides brasiliensis specific antibodies has great importance for indirect diagnosis of paracoccidioidomycosis (PCM), mainly through the adversity of the mycological examination, as the difficulty of access to the site of injury and / or time consuming to obtain results. In many cases it is the only evidence of a mycological nature of the infection. The Manual of Surveillance of Paracoccidioidomycosis of Sao Paulo recommended for patients with clinical suspected of the disease or following treatment, the use of three different serological tests: ELISA, double immunodiffusion in agarose gel (DI) and immunoblotting (IB). In the present work, we analyzed the performance in the serological diagnosis of PCM in two of these assays. We evaluated by DI and IB, 36 sera samples of patients with proven PCM and 49 sera samples from healthy donors from Hemocentro do Hospital das Clinicas de Botucatu, endemic area for PCM. The DI assay showed sensitivity of 81% (29 ⁄36) and the efficiency of this technique was 92% (78 ⁄85). The IB sensitivity was 94% (34 ⁄36) and the efficiency was 98% (83 ⁄85). Both methodologies showed specificity of 100%. The agreement analysis between these techniques performed by Cohen′s kappa index was considered good (k = 0. 76). Both methods showed high efficiency (more than 90%) and IB showed a greater ability to discriminate the positive samples, when it´s not possible by DI. In addition, the IB was able to determine the reactivity of specific antigenic fractions, such as gp43 and gp70. In turn, the DI, due to its semi-quantitative property, allows the measurement of titles of circulating antibodies that assists in interpreting the therapeutic response and as a serologically cure criterion. The immunoassays showed be complementary to the presumptive diagnosis, prognosis of the disease and possible early detection of relapses.਀㰀戀㸀匀甀瀀瀀漀爀琀攀搀 戀礀㰀⼀戀㸀㨀䤀渀猀琀椀琀甀琀漀 䄀搀漀氀昀漀 䰀甀琀稀 ⠀倀爀漀樀攀琀漀 䌀吀䌀ⴀ䤀䄀䰀 ⌀㈀㜀⼀ 㤀ⴀ䈀䴀⤀㰀⼀昀漀渀琀㸀㰀⼀瀀㸀㰀戀爀㸀㰀戀㸀䬀攀礀眀漀爀搀㨀 㰀⼀戀㸀☀渀戀猀瀀㬀䐀漀甀戀氀攀 椀洀洀甀渀漀搀椀昀昀甀猀椀漀渀Ⰰ 䤀洀洀甀渀漀愀猀猀愀礀猀Ⰰ 䤀洀洀甀渀漀戀氀漀琀琀椀渀最Ⰰ 䬀愀瀀瀀愀 椀渀搀攀砀Ⰰ 倀愀爀愀挀漀挀挀椀搀椀漀椀搀漀洀礀挀漀猀椀猀㰀⼀琀搀㸀㰀⼀琀爀㸀㰀⼀琀愀戀氀攀㸀㰀⼀琀爀㸀㰀⼀琀搀㸀㰀⼀琀愀戀氀攀㸀㰀⼀戀漀搀礀㸀㰀⼀栀琀洀氀㸀