DETECTION OF GIARDIA DUODENALIS GENOTYPE AII IN WELL WATER FROM A PERIURBAN COMMUNITY LOCATED IN SUZANO, SÃO PAULO.

Licia Natal Fernandes (FSP/USP); Patricia Pereira de Souza (FSP/USP); Ronalda Silva de Araújo (FSP/USP); Milena Dropa (FSP/USP); Lívia Carminato Balsalobre (FSP/USP); Kélvilin Anahí Gonzales Sábio (FSP/USP); Maria Tereza Peppe Razzolini (FSP/USP); Glavur Rogério Matté (FSP/USP); Maria Helena Matté (FSP/USP)

Giardia duodenalis is a protozoan that parasitizes humans and other mammals and can cause giardiasis. Although its isolates have been divided into seven Assemblages, named A to G, only A and B have been detected in human feces. Assemblege A isolates are commonly divided into two genotypes, AI and II. In Brazil there is scarce information about the presence of this protozoan in well water. As this matrice is used by many communities as source of water for human consumption, it is important to verify the presence of this parasite in these samples, and detect which assemblages might be present, which can only be done by the employment of molecular techniques. Three samples (10L) of well water were collected in periurban communities located in the municipality of Suzano (SP) and concentrated by modified membrane filtration technique. Two of the samples were also purified before DNA was extracted. A nested PCR that amplifies a 890bp of gdh gene was proceeded. Positive samples were cloned and sequenced. Phylogenetic analyse was developed by Mega 4.1 beta program. Two out of three well samples were confirmed to be positive for Giardia duodenalis. The only negative sample did not go through purification step which indicates that this might be important to remove PCR inhibitors, frequently present in water samples. Phylogenetic tree placed the sequences obtained in this work with the genotype AII sequences from Genbank used as reference strains. As this genotype is commonly associated to human giardiasis, the contact with the well water from these comunities represents a risk for public health. Therefore, the well water should undergo to some treatment process before its consumption and more attention should be given to this kind of water in order to avoid contamination. Apoio: Finep e CNPq.