The basidiomycetes Cryptococcus neoformans  is  an  encapsulated  pathogenic  fungus.  The cryptococcal capsule is composed of polysaccharides and is necessary for virulence.  Glucuronoxylomannan , the major capsular component, is synthesized in the cytoplasm and transported  to  the extracellular space in vesicles, but knowledge on the organelles  involved in polysaccharide  synthesis  and  traffic  is  extremely  limited. In  this  work  we  report the glucuronoxylomannan distribution in  Cryptococcus  neoformans cells sectioned by cryoultramicrotomy  and  visualized  by  transmission  electron  microscopy (TEM). The ultrathin sections were stained with the monoclonal antibody 18B7 which specifically binds to the capsular polysaccharide and followed  by  the  gold-labeled  anti- mouse IgG. Cryosections of fungal cells showed high preservation of intracellular  organelles  and  cell  wall structure. Incubation  of  cryosections  with  the  Mab18B7 revealed that cytoplasmic structures associated to vesicular compartments and reticular membranes are in close proximity to the polysaccharide. Glucuronoxylomannan  was generally found in association with intracellular lipid bilayers and within the cell wall matrix. Analysis of extracellular fractions from cryptococcal supernatants by thin layer chromatography revealed lipid containing fractions, some of which were associated with glucuronoxylomannan. In this work we developed a cryosectioning protocol for Cryptococcus neoformans, which allowed a unique resolution of the fungal cytoplasmic content and surface structures. These results indicate an intimate association of glucuronoxylomannan  and lipids in both intracellular and extracellular spaces consistent with polysaccharide synthesis and transport in membrane-associated structures.