Resumo

The infections of the urogenital tract of bacterial origin have a high incident between the feminine world population in reproductive age. A great proportion of these diseases, as the bacterial vaginosis (VB) they are caused often by multiple pathogenic, such agents as Gardnerella vaginalis, Mobiluncus and Bacteroides spp., Peptococcus, Peptoestreptococcus, Prevotella, Mycoplasma hominis. One strain of Lactobacillus, known as Lactobacillus sp. BMP9, was selected from among 320 strains isolated from vaginal swabs of between 8 healthy women with a score of zero according to Nugent’s criteria. Lactobacillus sp. BMP9 was chosen as potential probiotic against bacterial vaginosis, based on its higher production of hydrogen peroxide mainly (25 mg/L), incubated at 37°C for 48 hours in Man Rogosa Sharpe broth, determined by the enzymatic method Merkoquant® Peroxide Test. It was showed the antagonistic activity of Lactobacillus sp. BMP9 strain against Staphylococcus aureus through the spot-on-lawn method of Lewus and in the supernatant of lactobacillary culture by the growing inhibition zone and the no change in optical density of indicator strain, respectively. The effect of vaginal re-colonization by Lactobacillus sp. BMP9, at bedtime for 7 nights, in women diagnosed with bacterial vaginosis (BV) by Nugent test showed cure of 81,25% (13/16) of BV and in the normal women (Nugent: 3 points) it wasn’t observe negative effects of 100% (4/4) follow-up at day 30 post-treatment control (p<0,05) and Odds ratio 1,44. Lactobacillus sp. BMP9 strain could be a good alternative probiotic to the treatments and prophylaxis of BV and others genital infections to research. In the conditions of work exposed, we can conclude the following thing: There were obtained vaginal cultures of producing Lactobacillus sp. of 25 mg/L of H2O2 for the treatment of the bacterial vaginosis. The vine-stock Lactobacillus sp. BMP9 showed, in vitro, antagonistic activity against Staphylococcus aureus, disabling his growth. The vaginal resettling for Lactobacillus sp. BMP9 achieved 81,25 % of priest of VB and the normalization of 68,75 % in women with vaginosis bacterial.