XXI ALAM
Resumo:935-2


Poster (Painel)
935-2DNA alterations in alveolar macrophages in response to Histoplasma capsulatum biofilm
Autores:Nayla de Souza Pitangui (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Janaína de Cássia Orlandi Sardi (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Aline Raquel Voltan (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Fernanda Patrícia Gullo (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Felipe de Oliveira Souza (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Christiane Pienna Soares (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Gabriela Rodriguez-arellanes (UNAM - Universidade Nacional Autonoma do México) ; Maria Lúcia Taylor (UNAM - Universidade Nacional Autonoma do México) ; Maria José Soares Mendes-giannini (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho") ; Ana Marisa Fusco-almeida (UNESP - Universidade Estadual Paulista "Júlio de Mesquita Filho")

Resumo

Introduction: Histoplasma capsulatum var. capsulatum is a dimorphic fungus that causes histoplasmosis, a respiratory and systemic disease. H. capsulatum is primarily acquired via aerosol exposure with the inhalation of microconidia or hyphal fragments. Evolution of respiratory disease depends on the ability of Histoplasma yeast to survive and replicate within alveolar macrophages. Biofilms are dynamic communities of microorganisms persistently attached to biological and nonbiological surfaces that are enclosed in an exopolymeric matrix, a state that presumably allows microbial cells to both survive hostile environments and disperse to colonize new niches. Based on the potential importance of biofilms to its survival in the human host and in nature, this study was designed to investigate the biofilm formation by H. capsulatum and correlation with the infection pattern of H. capsulatum yeast in macrophages (AMJ2 - C11), using microscopic techniques, as well as establish the fungal ability to induce DNA damage in this cell line. Materials/Methods: Two H. capsulatum strains were used to form biofilms and infection assay: the EH 315 strain, which was isolated from the intestine of infected bats captured in the state of Guerrero, Mexico, and the 60I strain, which was isolated from mouth of a patient infected in the city of Araraquara, Brazil. For interaction testing we used a macrophage line, AMJ2–C11. The biofilm formation was evaluated by scanning electron microscopy (SEM). Interaction of both strains (EH-315 and 60I) to cell lineage was examined using IN Cell Analyzer 2000 System light microscopy. The damage was examined by comet assay. Discussion/Results: The results showed that H. capsulatum has a great ability to form biofilm and exhibited a particular characteristic in the interaction with macrophages. The fungi were directed to the nucleus, forming a “crown” yeast compact. The two strains tested, 60I and EH-315, induced DNA damage, respectively, 10,78 ± 1,31% and 10,67 ± 0,91% damage, showed significant difference to negative control (1,75 ± 0,18%). This is probably a mechanism of fungal adaptation to the host cell, since in this interaction, the fungus is able to survive and replicate in alveolar macrophages. Conclusion: H. capsulatum is able to form mature biofilm and was able to induce 10% DNA damage in interaction with macrophages.


Palavras-chave:  Alveolar macrophages, DNA damage, Histoplasma capsulatum