XXI ALAM
Resumo:591-2


Poster (Painel)
591-2Filamentous fungi from the rhizosphere of sugarcane in soil naturally infested with Meloidogyne sp.
Autores:Cláudia Elizabete Pereira Lima (UFPE - Universidade Federal de Pernambuco) ; Anna Karla Santana Silva (UFPE - Universidade Federal de Pernambuco) ; André Ferraz Goiana Leal (UFPE - Universidade Federal de Pernambuco) ; Romero Marinho Moura (UFPE - Universidade Federal de Pernambuco) ; Idjane Santana Oliveira (UFPE - Universidade Federal de Pernambuco)

Resumo

The rhizosphere is the site of intense microbial activity in which the organic nutrients coming from the roots favour the development of microorganisms. This study aimed to increase knowledge about the diversity of filamentous fungi in the rhizosphere of sugarcane, in soil naturally infested with Meloidogyne sp., in northeastern Brazil. Composite samples consisting of 10 subsamples from sugarcane rhizosphere soil were collected in the city of Carpina, PE, directly from two experimental sites, one infested with eggs and juveniles of plant parasitic nematode Meloidogyne sp. (area A) and the other with absence of Meloidogyne sp. (area B). Then the soil samples were diluted to 10-4, plated in culture medium agar Sabouraud (SAB) and incubated for 72 hours at room temperature (±28 °C). We estimated the colony-forming units (CFU) of filamentous fungi per gram of soil and the relative frequency of each gender in both areas. All isolated filamentous fungal strains were identified at the genus level according to macroscopic and microscopic characteristics. We obtained of anamorphic Ascomycota (Penicillium, Aspergillus, Fusarium, Trichoderma and Cladosporium). The largest number of isolates, as well as the relative frequency of occurrence of these fungi corresponded to genera Penicillium (44 and 57%) and Aspergillus (22 and 43%), these values represented in areas A and B, respectively. The total number of CFU per gram of soil samples varied depending on the collection area. A large number of fungal was observed in the area B (105 CFU.g-1 of soil) when compared to the area A, infested with phytonematodes (2 x 103 CFU.g-1 of soil). According to our results, the absence of Meloidogyne sp. in the area B, is probably due to the fungal predominence in the soil.


Palavras-chave:  Filamentous fungi, Meloidogyne, rhizosphere, sugarcane