Resumo

Introduction: Natural products have been a source of important drugs to treat infectious disease. Inga laurina (Fabaceae) is a tropical plant with distribution in Central America and South America and It has wide distribution in Brazil, occurring from the Amazon to the northeast and south to Paraná in almost all vegetation .Popularly known as white-inga, Inga-de-chichica, Inga-of-monkey, Inga-of-beach, Inga-Mirim or Ingaí. Candida infections are the cause of death in immunocompromised patients and immunocompetent. While C. albicans is still considered the most common species in candidemia, increased rates of candidemia caused by non-albicans species has been reported in Brazil and are commonly associated with cancer, neutropenia and the previous use of fluconazole. The traditional treatment with drugs such as fluconazole and amphotericin B may not be sufficient to treat bodies have an intrinsic resistance or acquired to these drugs, such as Candida glabrata and Candida krusei. Materials and Methods: The antifungal activity of ethanolic crude extract, fractions and phytochemicals was determined for Candida sp (Candida albicans (ATCC 64548), Candida krusei (ATCC6258), Candida parapsilosi (ATCC22019), Candida tropicalis (ATCC750) and Candida glabrata (ATCC90180)). For determination of antifungal activity the compounds was tested by the microdilution method in accordance with the M27-A2 (2002) of CSLI. Cytotoxicity was assessed by Sulforodhamina B in NOK (Normal oral keratynocyte) and C33A (Cervical carcinoma). Results: The extract and fractions hydromethanol, ethyl acetate, n-butanol, hexane and phytochemicals 1-rhamnopyranosyl-2-galoil-4,6-dyhydroxyphenyl , 1-rhamnopyranosyl-2-galoil-3,5-dyhydroxyphenyl/ and gallic acid showed fungistatic activity for Candida sp, good activity against non-albicans sp (MIC 1,97 at 7,8 µg/mL for extract and fractions, MIC 0,48 at 3,90 µg/mL for 1-rhamnopyranosyl-2-galoil-4,6-dyhydroxyphenyl and MIC 7,8 at 125 µg/mL for isomers) and It were not cytotoxic in NOK and C33A. The phytochemicals reveal good activity against Candida krusei (MIC 0,48 µg/mL) and Candida glabrata (MIC 0,97µg/mL). Discussion and Conclusion: The leaves of the species of Inga laurina are potential sources of bioactive compounds against fungi opportunistic or resistant to conventional drugs. Bolsa: CAPES