Resumo

In the last year the incidence of infections caused by fungi has increased considerably where dermatophytosis represents an important public health problem. In association this mycosis is often related to relapses after cessation of therapy. However, various antifungal agents including the triazoles, imidazole, allylamine and griseofulvin have been reported to have substantial activity. The determination of other compounds as antifungal potential may prove helpful to eradicate the dermatophytes. Despite the widespread prophylactic use of agents with antifungal properties, infections caused by pathogenic fungi are causing increasing resistance. High recurrence has sparked growing concern among clinicians regarding the potential emergence of among the species from dermathophyte. Considering the need of more effective and less toxic drugs, we evaluated clinical dermatophytes susceptibility against pure complex 2-[4-nitro-benzilidene)-amino]-4,5,6,7-tetrahidro-benzo[b]thiophene-3-carbonitrile and microemulsioned formulation. The minimal inhibitory concentration (MIC) was determined following the microdilution method M38-A2 indicated by the Clinical and Laboratory Standards Institute. The culture medium used was RPMI 1640 (Sigma-Aldrich, EUA) sterilized by membrane 0,22µm (Millipore, Darmstadt, Germany). The commercial antifungal agent used was amphotericin B (Bristol-Myers Squibb, Princeton, USA). To determine the minimum fungicidal concentration (MFC) of the thiophenes, the content of the wells that showed 100% inhibition of growth medium was cultured for Sabouraund-Glucose-Agar in Petri dishes. All isolates evaluated presented MIC to thiophene microemulsioned, which showed antifungal activity among 0,10-83,33μg/mL against the dermatophytes Trichophyton mentagrophytes, T. rubrum, T tonsurans and Microsporum canis isolates. The all isolates studied were resistant to pure thiophene. This paper underscores the importance of the antifungal potential of thiophene derivates against dermatophytes an alternative treatment against fungal infections. However, in vivo experiments are essential for future medical use.