Poster (Painel)
| 1533-2 | Detection of carbapenemase encoding genes in Pseudomonas spp and Acinetobacer spp from samples of municipal wastewater treatment plant and effluent of a hospital where the SPM-1 metallo-beta-lactamase and OXA-23 carbapenemase are endemic. | | Autores: | Palermo, R.L. (UNIFIL - Centro Universitário Filadélfia) ; Romanin, P. (UNIFIL - Centro Universitário Filadélfia) ; Shibayama-Fernandes, M.M. (UNOPAR - Universidade Norte do Paraná) ; Santos, L.L (UNOPAR - Universidade Norte do Paraná) ; Pitz, A.F. (UEL - Universidade Estadual de Londrina) ; Pelisson, M. (UEL - Universidade Estadual de Londrina) ; Perugini, M.R.E (UEL - Universidade Estadual de Londrina) ; Venancio, E.J. (UEL - Universidade Estadual de Londrina) ; Carrara-Marroni, F.E. (UEL - Universidade Estadual de Londrina) |
Resumo Metallo-beta-lactamases- (MBL) and OXA-carbapenemases- (OXA) encoding genes are an important source of acquired carbapenem resistance in Non-fermenting Gram negative bacilli (NFGNB). Durant last years, Pseudomonas aeruginosa producing SPM-1 metallo-beta-lactamase and OXA-23-producing Acinetobacter baumannii has been considered endemic in Hospital Universitário de Londrina (HU). The blaSPM-1/blaIMP-16 and blaOXA-23/blaOXA-143 genes were detected, respectively, in clinical isolates of Pseudomonas aeruginosa and Acinetobacter baumannii from this hospital. Since the effluent at the HU do not receive treatment before to be discharged into the municipal wastewater network, we conducted a study in order to detect the presence of NFGNB carrying carbapenemases genes in different sewage related to this hospital. Were collected samples from non-treated HU effluents, municipal Wastewater Treatment Plant (WWTP) that receive this hospital sewage, and from superficial water of the adjacent river where the effluent treated are discharged. A total of twelve Pseudomonas spp and nine Acinetobacter spp isolates with reduced susceptibility to imipenem and or meropenem were screened for the presence of genes: blaIMP, blaVIM, blaSPM, blaGIM, blaSIM, blaOXA-23, blaOXA-24, blaOXA-51, blaOXA58 and blaOXA-143 by PCR multiplex as previously described. A total of six isolates of Pseudomonas spp showed MBL genes, three of them from sewage inflow WWTP and HU effluent were positive for the blaIMP gene and more three isolates from effluent of WWTP showed the blaSPM gene. Nine isolates of Acinetobacter spp carried the blaOXA-51/blaOXA-23 genes and these isolates were detected in all samples investigated including that water river. All the NFGNB carrying the carbapenemases genes were carbapenemase producers by the Hodge test. These results indicate that MBL and OXA resistance genes have already crossed hospital limits and reinforce the possible role of the aquatic environment as a reservoir for carbapenemase genes. Additional studies are being conducted to determine the genetic relatedness of isolates obtained from different sewage samples. |