Resumo

Cellulose is regarded as one of the only renewable carbon, and is available in large quantities cellulosic residues, accounting for over 60% of agricultural waste. The cellulose is enzymatically hydrolyzed by cellulase, which does not correspond to a single enzyme, but the synergistic action of endoglucanases and exoglucanases synthesized by micro-organisms.The cellulase enzymes are of economic importance and are sold in large volumes with various industrial applications, such as in starch processing, animal feed production, fermentation of grain to ethanol production, extraction of fruit juice and vegetable pulp industry paper and textile industry. The experiment was conducted at the Laboratory of General Microbiology, Center for Agricultural Sciences, Federal University of Alagoas. The soil was collected and adicoinaded 10g on frask containing saline solution to decimal serial dilution of 10 ^-1 to 10 ^-4. Aliquots of each dilution were pipetted into a Petri dish containing medium and incubated until Martin colony growth. Isolates that showed potential were evaluated for cellulosic pulp production totals, using the technique of halo formation of degradation. The strains were incubated in test tubes containing 15 x 2.5 cm cellulose acidic medium, incubated at 28 ºC.Os hydrolysis halos were measured depth and subjected to analysis of variance. Were obtained using UFCg Martin 3.22 x 10 2 -1, of which nine isolates had basic characteristic, that is ability to grow in culture medium, where the only carbon source is cellulose, the primary method selected paper filter. Eight showed the production of halo hydrolysis causing the enzymatic action to change the color of the culture medium opaque to translúcido.Com hydrolysis ratio halo, analysis of variance detected significant differences among the isolates. The interest in filamentous fungi is due to its ability to cleave different substrates, which is exploited generating products or procedures, such as isolation of enzymes, biodegradation of waste and solid state fermentation of waste. The best results were obtained cellulase production by isolates 1 and 7.