Poster (Painel)
| 1002-1 | ESBL in clinical Klebsiella pneumoniae isolates from dogs in Brazil | | Autores: | Pardinho, A.M. (UNICAMP - Universidade Estadual de Campinas) ; Macedo, G.G. (UNICAMP - Universidade Estadual de Campinas) ; Lara, G.H.B. (UNESP - Universidade Estadual Paulista) ; Franco, M.M.J. (UNESP - Universidade Estadual Paulista) ; Listoni, F.J.P. (UNESP - Universidade Estadual Paulista) ; Ferraz, M.M.G. (UNICAMP - Universidade Estadual de Campinas) ; Paes, A.C. (UNESP - Universidade Estadual Paulista) ; Ribeiro, M.G. (UNESP - Universidade Estadual Paulista) ; Leite, D.S. (UNICAMP - Universidade Estadual de Campinas) ; Siqueira, A.K. (UNICAMP - Universidade Estadual de Campinas) |
Resumo Klebsiella pneumoniae are opportunistic pathogens of humans and animals. These pathogens are found in intestinal microbiota from pet animals and can be associated with clinically significant infections of the gastrointestinal tract and with a wide range of infections, such as hemorrhagic gastroenteritis and septicemia. K. pneumoniae remains as one of the extended-spectrum β-lactamase (ESBL)-producing microorganisms isolated. In general, pet animals live as individuals in intimate contact with their owners enabling the spread of multiresistant bacteria between owners and other animals with high efficiency. The aim of this study was to describe the presence of two ESBL-producing K. pneumoniae recovered from fecal samples collected from two different dogs from different houses and owners with enteric signs and sepsis in Botucatu city, São Paulo state, Brazil. K. pneumoniae strains were identified using biochemical and molecular tests. The isolates were submitted to the disc diffusion method and double disc approximation test according to CLSI criteria. PCR analyses were performed to determine the resistant genes carried by isolate. The two strains were resistant to aminoglycosides, aminopenicillins, cephalosporins, chloramphenicol, fluorquinolones, trimethoprim/sulfamethoxazole and tetracycline and presented a double disc approximation test positive for ESBL production. PCR revealed that the strains possessed SHV and TEM β-lactamases. Historically the most common ESBLs have been TEM and SHV types. The use of β-lactams in veterinary medicine could be a reason to the high ESBL occurrence worldwide. The isolation of ESBL-producing microorganisms is the main cause of therapeutic failure, leading to increased considerable morbidity and mortality by bacterial infections. This fact indicate that continuous monitoring systems and effective control measures are absolutely necessary to prevent and/or avoid the dissemination of these extended-spectrum β-lactamase (ESBL)-producing microorganisms between humans and animals.
Fapesp processos 2011/17957-0 e 2012/03128-5. |