Resumo

More than 100 HPV genotypes have been identified so far, and about 20 of them are associated to severe cervical neoplasia. The goal of the present study was to trace the spectrum of HPV types in Rio de Janeiro state, and HPV prevalence according to cytological status. A cross-sectional study population included 891 infected women whose have taken cervical smears from 2003 to 2010. Data about these women were recorded from a data bank. The samples were analyzed in other studies which were approved by Ethical Committee of the College of Medicine of the Universidade Federal Fluminense. No patient was sampled solely for the purpose of our work. According to the Bethesda nomenclature, cervical smears were classified as normal/inflammatory, atypical squamous cells of undetermined significance (ASCUS), low grade squamous intraepithelial lesions (LSIL), high grade squamous intraepithelial lesions (HSIL), and squamous carcinoma (CA). MY09/11 consensus primers were used to detect generic HPV DNA. HPV typing was performed by restriction fragment length polymorphism analysis (RFLP) following polymerase chain reaction (PCR) amplification. The patients aged 14-79 years. The mean age was 31.8 years, standard deviation 13.0 years. Most women (673) presented normal cytology (75.5%), 28 ASCUS (3.1%), 97 LSIL (10.9%), 60 HSIL (6.7%), 29 CA (3.3%) and 4 were inconclusive (0.4%). The overall HPV prevalence was 32.8% (291/891). The frequency of HPV infection to each cytological category was: normal (19%), ASCUS (35.7%), LSIL (80.4%), HSIL (78.3%), and CA (96.6%). We identified 33 different HPV genotypes. The HPV 16 was the most prevalent type (9.1%), followed by HPV 6 (3.4%), 18 (2.4%), and 31 (2.0%). Among the high risk HPV types for cervical cancer, 15 were found in prevalence lower than 2.0%. , as well as another 14 low risk HPV types which ranged from 0.1% to 1.5%. The types 26, 35, 51, 69, s82 (high risk), and 32, 40, 44, 54, 62, 70, 83, 84, and LX100 (low risk) were found in frequency so low that we speculate if they were circulating in a short time in this geographic area, with no important clinical outcome, being cleared from the body by the immune system. By other side, they could have low tropism for the host. These types were not associated with severe neoplasia. So, 14 HPV types found in this work could be seen as temporary infections, without harmful potential. Financial support: PROPPi-UFF, CNPq, FAPERJ