Resumo

Introduction and Objectives: Silver nanoparticles (SN) are nanoparticles of silver, i.e. silver particles of between 1 nm and 100 nm in size. SN have antimicrobial character, and when coated in the porous membranes of poly (lactic-co-glycolic acid) or PLGA. PLGA is a copolymer which is used in a host of Food and Drug Administration (FDA) approved therapeutic devices, owing to its biodegradability and biocompatibility. The complex PLGA-SN have a great potential therapeutic for scaffolds applications in skin infections combining two components into a single material with bioactive properties. Material and Methods: The assays were carried out with PLGA membranes immersed in a solution of silver nanoparticles (SN). SN were stabilized in solutions containing: PVP/Pluronic, PVA/Pluronic, PVP, PVA and citrate. The solutions containing SN were added in tubes on the presence of PLGA, solutions remained in contact with the membranes at 30.0ºC and 150 rpm at until its complete drying. The inhibitory activity of SN in PLGA and residual solutions were evaluated in agar diffusion assays (in mm) utilizing P. aeuroginosa, E. coli, and S. aureus, at 37.0ºC during 24 hours. Results and Conclusions: According to the results obtained against P. aeuroginosa, all solutions containing SN showed inhibition halos of 18.00 mm, with a peak for PVA solution (21.70 mm). Solutions against E. coli showed inhibition halos of 12.00 mm, an effect not observed for PVP solution. Moreover, against E. coli SN stabilized with PVA again had the highest effect (15.00 mm). However, solutions containing SN showed no inhibitory activity against S. aureus. On the other hand, this effect does not invalidate the results obtained, because the complex PLGA-SN is non-toxic, biocompatible, and present great potential in the field of medical microbiology.